Recombinant Rat Inward rectifier potassium channel 16 (Kcnj16) Protein, Rattus norvegicus (Rat), in vitro E.coli expression system, N-terminal 10xHis-tagged, 54.0kDa, purity >85% as determined by SDS-PAGE.

Recombinant Rat Inward rectifier potassium channel 16 (Kcnj16) Protein

Recombinant Rat Inward rectifier potassium channel 16 (Kcnj16) Protein — Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Purity >85%.

SKU: BCRECP-00129TM Category:
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Product Specifications

Uniprot No.P52191
Gene NamesKcnj16
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-419aa
SpeciesRattus norvegicus (Rat)
Tag InfoN-terminal 10xHis-tagged
Molecular weight54.0kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.

Biological Context

Subcellular Location: Membrane (Multi-pass membrane protein); Basolateral cell membrane
Tissue Specificity: Expressed in the brain, testis, liver, spleen, kidney, submaxillary gland and adrenals. In the kidney, expressed in the epithelial cells of both proximal and distal convoluted tubules, in the endothelial cells surrounding glomerular capillaries and in the flattened parietal layer of Bowman's capsule

Product Specifications

Recombinant Rat Inward rectifier potassium channel 16 (Kcnj16) Protein is a recombinant protein from Rattus norvegicus (Rat), expressed in in vitro E.coli expression system, covering amino acids 1-419aa, with N-terminal 10xHis-tagged tag, molecular weight 54.0kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. KCNJ16 may be involved in the regulation of fluid and pH balance (By similarity). In the kidney, together with KCNJ10, mediates basolateral K(+) recycling in distal tubules; this process is critical for Na(+) reabsorption at the tubules

Subcellular Location

Membrane (Multi-pass membrane protein); Basolateral cell membrane

Tissue Specificity

Expressed in the brain, testis, liver, spleen, kidney, submaxillary gland and adrenals. In the kidney, expressed in the epithelial cells of both proximal and distal convoluted tubules, in the endothelial cells surrounding glomerular capillaries and in the flattened parietal layer of Bowman's capsule

Subunit

It forms heteromeric channels with Kir4.1/KCNJ10; this interaction is required for KCNJ16 localization to the basolateral membrane in kidney cells. As a heteromer with KCNJ10, may interact with MAGI1; this interaction may facilitate KCNJ10/KCNJ16 potassium channel expression at the basolateral membrane in kidney cells. May form heteromers with Kir2.1/KCNJ2 (By similarity). Can form heteromeric channels with Kir4.2/KCNJ15 (By similarity)

Gene: Kcnj16  |  Organism: Rattus norvegicus  |  Synonyms: BIR9; Inward rectifier K(+) channel Kir5.1; Potassium channel, inwardly rectifying subfamily J member 16

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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