Recombinant Rat Klotho (Kl), partial, Rattus norvegicus (Rat), E.coli, N-terminal 6xHis-tagged, 64.9kDa, purity >85% as determined by SDS-PAGE.

Recombinant Rat Klotho (Kl), partial

Recombinant Rat Klotho (Kl), partial — recombinant protein from Rattus norvegicus (Rat), expressed in E.coli. Purity >85%.

SKU: BCRECP-003215 Category:
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Product Specifications

Product SkuBCRECP-003215
Product DescriptionRecombinant Rat Klotho (Kl) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 35-550aa. [Accession | Q9Z2Y9].
Uniprot No.Q9Z2Y9
Gene NamesKl
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region35-550aa
SpeciesRattus norvegicus (Rat)
Tag InfoN-terminal 6xHis-tagged
Molecular weight64.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Present in cortical renal tubules and the parathyroid (at protein level). Strongly expressed in kidney. Expressed at low levels in brain, lung, intestine and ovaries

Product Specifications

Recombinant Rat Klotho (Kl), partial is a recombinant protein from Rattus norvegicus (Rat), expressed in E.coli, covering amino acids 35-550aa, with N-terminal 6xHis-tagged tag, molecular weight 64.9kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling

Subcellular Location

Secreted

Tissue Specificity

Present in cortical renal tubules and the parathyroid (at protein level). Strongly expressed in kidney. Expressed at low levels in brain, lung, intestine and ovaries

Subunit

Homodimer. Interacts with FGF23 and FGFR1

Gene: Kl  |  Organism: Rattus norvegicus
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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