Recombinant Rat Melanopsin (Opn4) Protein, Rattus norvegicus (Rat), in vitro E.coli expression system, N-terminal 10xHis-tagged, 53.9kDa, purity >85% as determined by SDS-PAGE.

Recombinant Rat Melanopsin (Opn4) Protein

Recombinant Rat Melanopsin (Opn4) Protein — G protein-coupled photoreceptor that signals through the G(q/11) family of G proteins in response to light in intrinsically photosensitive retinal ganglion cells (ipRGCs). Purity >85%.

SKU: BCRECP-00263TM Category:
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Product Specifications

Uniprot No.Q8R456
Gene NamesOpn4
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-474aa
SpeciesRattus norvegicus (Rat)
Tag InfoN-terminal 10xHis-tagged
Molecular weight53.9kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

G protein-coupled photoreceptor that signals through the G(q/11) family of G proteins in response to light in intrinsically photosensitive retinal ganglion cells (ipRGCs). Can also activate other G proteins, including G(i/o) and G(s), depending on the cell type, leading to either activation or inhibition of adenylate cyclase and cAMP-dependent signaling. Activation occurs when opsin-bound cis-retinal chromophore absorbs a photon and isomerizes to all-trans-retinal, inducing a conformational change in the opsin that triggers a G protein-mediated phototransduction cascade.

Biological Context

Subcellular Location: Cell membrane (Multi-pass membrane protein); Cell projection, axon; Cell projection, dendrite; Perikaryon
Tissue Specificity: Eye; expressed in a photosensitive subset of retinal ganglion cells (at protein level)

Product Specifications

Recombinant Rat Melanopsin (Opn4) Protein is a recombinant protein from Rattus norvegicus (Rat), expressed in in vitro E.coli expression system, covering amino acids 1-474aa, with N-terminal 10xHis-tagged tag, molecular weight 53.9kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

G protein-coupled photoreceptor that signals through the G(q/11) family of G proteins in response to light in intrinsically photosensitive retinal ganglion cells (ipRGCs). Can also activate other G proteins, including G(i/o) and G(s), depending on the cell type, leading to either activation or inhibition of adenylate cyclase and cAMP-dependent signaling. Activation occurs when opsin-bound cis-retinal chromophore absorbs a photon and isomerizes to all-trans-retinal, inducing a conformational change in the opsin that triggers a G protein-mediated phototransduction cascade (By similarity). Acts as a bistable protein, allowing photoreversible regeneration of the cis-retinal chromophore after stimulation (By similarity). Contributes to non-image-forming visual response to light in ipRGCs, including the pupillary light reflex and the regulation of circadian rhythms. In M1 ipRGCs (non-image forming) subtypes, activates a G(q)-phospholipase C (PLC) signaling cascade leading to depolarization through opening of TRPC6/7 channels and decreased intrisic excitability. Mediates phototransduction in M4 ipRGCs (image forming) subtypes across dim to bright light conditions via G(q)-PLC signaling, resulting in increased intrisic excitability through closure of potassium leak channels (most likely TASK channels), thereby enhancing contrast sensitivity. At bright light intensities, further enhances depolarization in M4 ipRGCs via activation of TRPC channels. Functions in the iris to control the intrinsic pupillary light reflex in nocturnal/crepuscular non-primate mammals. May be involved in the optokinetic visual tracking response. May also play a role in regulating retinal hyaloid vessel growth and regression (By similarity)

Subcellular Location

Cell membrane (Multi-pass membrane protein); Cell projection, axon; Cell projection, dendrite; Perikaryon

Tissue Specificity

Eye; expressed in a photosensitive subset of retinal ganglion cells (at protein level)

Gene: Opn4  |  Organism: Rattus norvegicus  |  Synonyms: Opsin-4

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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