Recombinant Rat Netrin-1 (Ntn1) (X544S,X576S,X589S), Rattus norvegicus (Rat), E.coli, N-terminal 6xHis-tagged, 68.1kDa, purity >85% as determined by SDS-PAGE.

Recombinant Rat Netrin-1 (Ntn1) (X544S,X576S,X589S)

Recombinant Rat Netrin-1 (Ntn1) (X544S,X576S,X589S) — Netrins control guidance of CNS commissural axons and peripheral motor axons. Purity >85%.

SKU: BCRECP-003916 Category:
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Product Specifications

Product SkuBCRECP-003916
Product DescriptionRecombinant Rat Netrin-1 (Ntn1) (X544S,X576S,X589S) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 25-604aa(X544S,X576S,X589S). [Accession | Q924Z9].
Uniprot No.Q924Z9
Gene NamesNtn1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region25-604aa(X544S,X576S,X589S)
SpeciesRattus norvegicus (Rat)
Tag InfoN-terminal 6xHis-tagged
Molecular weight68.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasNeuroscience

Function

Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. Binding to UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion.

Biological Context

Subcellular Location: Secreted; Cytoplasm

Product Specifications

Recombinant Rat Netrin-1 (Ntn1) (X544S,X576S,X589S) is a recombinant protein from Rattus norvegicus (Rat), expressed in E.coli, covering amino acids 25-604aa(X544S,X576S,X589S), with N-terminal 6xHis-tagged tag, molecular weight 68.1kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. Binding to UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (By similarity). Involved in dorsal root ganglion axon projection towards the spinal cord (By similarity). It also serves as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in colorectal tumorigenesis by regulating apoptosis (By similarity)

Subcellular Location

Secreted; Cytoplasm

Subunit

Binds to its receptors; DCC, UNC5A, UNC5B, UNC5C and probably UNC5D. Binds to its receptor; DSCAM (By similarity). Interacts with APP (By similarity)

Gene: Ntn1  |  Organism: Rattus norvegicus

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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