Recombinant Rat Parathyroid hormone/parathyroid hormone-related peptide receptor (Pth1r), partial (Active), Rattus norvegicus (Rat), Mammalian cell, C-terminal 10xHis-tagged, 20.0 kDa, purity >90% as determined by SDS-PAGE.

Recombinant Rat Parathyroid hormone/parathyroid hormone-related peptide receptor (Pth1r), partial (Active)

Recombinant Rat Parathyroid hormone/parathyroid hormone-related peptide receptor (Pth1r), partial (Active) — G protein-coupled receptor for parathyroid hormone (PTH) and for parathyroid hormone-related peptide (PTHLH). Purity >90%.

SKU: BCACP-000318 Category:
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Product SKUBCACP-000318
Product DescriptionRecombinant Rat Parathyroid hormone/parathyroid hormone-related peptide receptor (Pth1r) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 27-188aa. [Accession | P25961].
Uniprot No.P25961
Gene NamesPth1r
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region27-188aa
SpeciesRattus norvegicus (Rat)
Tag InfoC-terminal 10xHis-tagged
Molecular weight20.0 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human GPR20 at 10 μg/mL can bind Anti-GPR20 recombinant antibody. The EC50 is 2.431 - 3.239 ng/mL.The VLPs is negative control.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesParathyroid hormone/parathyroid hormone-related peptide receptor; PTH/PTHrP type I receptor (PTH/PTHr receptor); Parathyroid hormone 1 receptor (PTH1 receptor); Pth1r; Pthr, Pthr1

Function

G protein-coupled receptor for parathyroid hormone (PTH) and for parathyroid hormone-related peptide (PTHLH). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (cAMP). PTH1R is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity.

Biological Context

Subcellular Location: Cell membrane (Multi-pass membrane protein)

Product Specifications

Recombinant Rat Parathyroid hormone/parathyroid hormone-related peptide receptor (Pth1r), partial (Active) is a recombinant protein from Rattus norvegicus (Rat), expressed in Mammalian cell, covering amino acids 27-188aa, with C-terminal 10xHis-tagged tag, molecular weight 20.0 kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

G protein-coupled receptor for parathyroid hormone (PTH) and for parathyroid hormone-related peptide (PTHLH) (PubMed:1313566). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (cAMP) (By similarity). PTH1R is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity (By similarity). PTHLH dissociates from PTH1R more rapidly than PTH; as consequence, the cAMP response induced by PTHLH decays faster than the response induced by PTH (By similarity)

Subcellular Location

Cell membrane (Multi-pass membrane protein)

Subunit

Homodimer in the absence of bound ligand. Peptide hormone binding leads to dissociation of the homodimer

Gene: Pth1r  |  Organism: Rattus norvegicus  |  Synonyms: PTH/PTHrP type I receptor; Parathyroid hormone 1 receptor

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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