Recombinant Rat Protransforming growth factor alpha (Tgfa), Rattus norvegicus (Rat), E.coli, N-terminal 6xHis-KSI-tagged, 30.1kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Rat Protransforming growth factor alpha (Tgfa)

Recombinant Rat Protransforming growth factor alpha (Tgfa) — recombinant protein from Rattus norvegicus (Rat), expressed in E.coli. Purity >90%.

SKU: BCRECP-004551 Categories: ,
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Product Specifications

Product SkuBCRECP-004551
Product DescriptionRecombinant Rat Protransforming growth factor alpha (Tgfa) Protein is expressed from E.coli with N-terminal 6xHis-KSI-tagged. It contains 24-159aa. [Accession | P01134].
Uniprot No.P01134
Gene NamesTgfa
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region24-159aa
SpeciesRattus norvegicus (Rat)
Tag InfoN-terminal 6xHis-KSI-tagged
Molecular weight30.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

Biological Context

Subcellular Location: Cell membrane (Single-pass type I membrane protein)

Product Specifications

Recombinant Rat Protransforming growth factor alpha (Tgfa) is a recombinant protein from Rattus norvegicus (Rat), expressed in E.coli, covering amino acids 24-159aa, with N-terminal 6xHis-KSI-tagged tag, molecular weight 30.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar

Subcellular Location

Cell membrane (Single-pass type I membrane protein)

Subunit

Interacts with the PDZ domains of MAGI3, SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2 (By similarity)

Gene: Tgfa  |  Organism: Rattus norvegicus

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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