Recombinant Rat Thyroglobulin (Tg), partial, Rattus norvegicus (Rat), E.coli, N-terminal 6xHis-SUMO-tagged, 48.0kDa, purity >90% as determined by SDS-PAGE.

Recombinant Rat Thyroglobulin (Tg), partial

Recombinant Rat Thyroglobulin (Tg), partial — Acts as a substrate for the production of iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). Purity >90%.

SKU: BCRECP-004550 Category:
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Product Specifications

Product SkuBCRECP-004550
Product DescriptionRecombinant Rat Thyroglobulin (Tg) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 21-300aa. [Accession | P06882].
Uniprot No.P06882
Gene NamesTg
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region21-300aa
SpeciesRattus norvegicus (Rat)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight48.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Acts as a substrate for the production of iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). The synthesis of T3 and T4 involves iodination of selected tyrosine residues of TG/thyroglobulin followed by their oxidative coupling. Following TG re-internalization and lysosomal-mediated proteolysis, T3 and T4 are released from the polypeptide backbone leading to their secretion into the bloodstream.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Specifically expressed in the thyroid gland
Disease Association: [Defects in Tg are a cause of a form of hypothyroidism in rdw rat]

Product Specifications

Recombinant Rat Thyroglobulin (Tg), partial is a recombinant protein from Rattus norvegicus (Rat), expressed in E.coli, covering amino acids 21-300aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 48.0kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Acts as a substrate for the production of iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3) (By similarity). The synthesis of T3 and T4 involves iodination of selected tyrosine residues of TG/thyroglobulin followed by their oxidative coupling (By similarity). Following TG re-internalization and lysosomal-mediated proteolysis, T3 and T4 are released from the polypeptide backbone leading to their secretion into the bloodstream (By similarity). One dimer produces 7 thyroid hormone molecules (By similarity)

Subcellular Location

Secreted

Disease Association

[Defects in Tg are a cause of a form of hypothyroidism in rdw rat]

Tissue Specificity

Specifically expressed in the thyroid gland

Subunit

Monomer (By similarity). Homodimer (via ChEL region); occurs in the endoplasmic reticulum and is required for export to the Golgi apparatus (By similarity). Homooligomer; disulfide-linked; stored in this form in the thyroid follicle lumen (By similarity)

Gene: Tg  |  Organism: Rattus norvegicus

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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