Recombinant Ross river virus Structural polyprotein, partial Protein, Ross river virus (strain T48) (RRV), in vitro E.coli expression system, N-terminal 10xHis-tagged, 50.3kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Ross river virus Structural polyprotein, partial Protein

Recombinant Ross river virus Structural polyprotein, partial Protein — Class II viral fusion protein. Purity >85%.

SKU: BCRECP-00141TM Categories: ,
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Product Specifications

Uniprot No.P08491
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region817-1254aa
SpeciesRoss river virus (strain T48) (RRV)
Tag InfoN-terminal 10xHis-tagged
Molecular weight50.3kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Class II viral fusion protein. Fusion activity is inactive as long as E1 is bound to E2 in mature virion. After virus attachment to target cell via host MXRA8 and endocytosis, acidification of the endosome induce dissociation of E1/E2 heterodimer and concomitant trimerization of the E1 subunits.

Biological Context

Subcellular Location: Virion membrane (Single-pass type I membrane protein); Host cell membrane (Single-pass type I membrane protein)

Product Specifications

Recombinant Ross river virus Structural polyprotein, partial Protein is a recombinant protein from Ross river virus (strain T48) (RRV), expressed in in vitro E.coli expression system, covering amino acids 817-1254aa, with N-terminal 10xHis-tagged tag, molecular weight 50.3kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Not implied unless activity data are explicitly listed on this product page, the datasheet, or the CoA.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Class II viral fusion protein. Fusion activity is inactive as long as E1 is bound to E2 in mature virion. After virus attachment to target cell via host MXRA8 and endocytosis, acidification of the endosome induce dissociation of E1/E2 heterodimer and concomitant trimerization of the E1 subunits. This E1 trimer is fusion active, and promotes release of viral nucleocapsid in cytoplasm after endosome and viral membrane fusion. Efficient fusion requires the presence of cholesterol and sphingolipid in the target membrane

Subcellular Location

Virion membrane (Single-pass type I membrane protein); Host cell membrane (Single-pass type I membrane protein)

Subunit

Oligomer (By similarity). Interacts with spike glycoprotein E1. Interacts with spike glycoprotein E2 (By similarity)

Organism: Ross river virus (strain T48)  |  Synonyms: p130
Key Publications

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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