Recombinant Rotavirus A Intermediate capsid protein VP6, Rotavirus A (strain RVA/Human/United States/Wa/1974/G1P1A[8]) (RV-A), Baculovirus, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 48.8kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Rotavirus A Intermediate capsid protein VP6

Recombinant Rotavirus A Intermediate capsid protein VP6 — Intermediate capsid protein that self assembles to form an icosahedral capsid with a T=13 symmetry, which consists of 230 trimers of VP6, with channels at each of its five-fold vertices. Purity >85%.

SKU: BCRECP-003514 Categories: ,
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Product Specifications

Product SkuBCRECP-003514
Product DescriptionRecombinant Rotavirus A Intermediate capsid protein VP6 Protein is expressed from Baculovirus with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-397aa. [Accession | P03530].
Uniprot No.P03530
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemBaculovirus
Expression Region1-397aa
SpeciesRotavirus A (strain RVA/Human/United States/Wa/1974/G1P1A[8]) (RV-A)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight48.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Intermediate capsid protein that self assembles to form an icosahedral capsid with a T=13 symmetry, which consists of 230 trimers of VP6, with channels at each of its five-fold vertices. This capsid constitutes the middle concentric layer of the viral mature particle.

Product Specifications

Recombinant Rotavirus A Intermediate capsid protein VP6 — Intermediate capsid protein that self assembles to form an icosahedral capsid with a T=13 symmetry, which consists of 230 trimers of VP6, with channels at each of its five-fold vertices. Purity >85%. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Intermediate capsid protein that self assembles to form an icosahedral capsid with a T=13 symmetry, which consists of 230 trimers of VP6, with channels at each of its five-fold vertices. This capsid constitutes the middle concentric layer of the viral mature particle. The innermost VP2 capsid and the intermediate VP6 capsid remain intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nascent transcripts are transcribed within the structural confines of this double-layered particle (DLP) and are extruded through the channels at the five-fold axes. VP6 is required for the transcription activity of the DLP

Subcellular Location

Virion

Subunit

Homotrimer. Interacts with the inner capsid protein VP2. Interacts with the outer capsid glycoprotein VP7. Interacts with the outer capsid protein VP5*

Organism: Rotavirus A (strain RVA/Human/United States/Wa/1974/G1P1A[8])

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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