Recombinant Rotavirus A Outer capsid glycoprotein VP7, Rotavirus A (isolate RVA/Cow/Thailand/A44/1988/G10P8[11]) (RV-A), Yeast, N-terminal 6xHis-tagged, 33.3kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Rotavirus A Outer capsid glycoprotein VP7

Recombinant Rotavirus A Outer capsid glycoprotein VP7 — Calcium-binding protein that interacts with rotavirus cell receptors once the initial attachment by VP4 has been achieved. Purity >90%.

SKU: BCRECP-003792 Categories: ,
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Product Specifications

Product SkuBCRECP-003792
Product DescriptionRecombinant Rotavirus A Outer capsid glycoprotein VP7 Protein is expressed from Yeast with N-terminal 6xHis-tagged. It contains 51-326aa. [Accession | Q00253].
Uniprot No.Q00253
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region51-326aa
SpeciesRotavirus A (isolate RVA/Cow/Thailand/A44/1988/G10P8[11]) (RV-A)
Tag InfoN-terminal 6xHis-tagged
Molecular weight33.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Calcium-binding protein that interacts with rotavirus cell receptors once the initial attachment by VP4 has been achieved. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. Following entry into the host cell, low intracellular or intravesicular Ca(2+) concentration probably causes the calcium-stabilized VP7 trimers to dissociate from the virion.

Biological Context

Subcellular Location: Virion; Host endoplasmic reticulum lumen

Product Specifications

Recombinant Rotavirus A Outer capsid glycoprotein VP7 is a recombinant protein from Rotavirus A (isolate RVA/Cow/Thailand/A44/1988/G10P8[11]) (RV-A), expressed in Yeast, covering amino acids 51-326aa, with N-terminal 6xHis-tagged tag, molecular weight 33.3kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Calcium-binding protein that interacts with rotavirus cell receptors once the initial attachment by VP4 has been achieved. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. Following entry into the host cell, low intracellular or intravesicular Ca(2+) concentration probably causes the calcium-stabilized VP7 trimers to dissociate from the virion. This step is probably necessary for the membrane-disrupting entry step and the release of VP4, which is locked onto the virion by VP7

Subcellular Location

Virion; Host endoplasmic reticulum lumen

Subunit

Homotrimer; disulfide-linked. 2 Ca(2+) ions bound at each subunit interface in the trimer hold the trimer together. Interacts with the intermediate capsid protein VP6. Interacts with the outer capsid protein VP5*

Organism: Rotavirus A (isolate RVA/Cow/Thailand/A44/1988/G10P8[11])

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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