Recombinant Rotavirus X Outer capsid protein VP4, partial, Rotavirus X (isolate RVX/Human/Bangladesh/NADRV-B219/2002/GXP[X]) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-B219)), Yeast, N-terminal 6xHis-tagged, 30.8kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Rotavirus X Outer capsid protein VP4, partial

Recombinant Rotavirus X Outer capsid protein VP4, partial — Forms the head of the spikes and mediates the recognition of specific host cell surface glycans. Purity >85%.

SKU: BCRECP-003825 Categories: , ,
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Product Specifications

Product SkuBCRECP-003825
Product DescriptionRecombinant Rotavirus X Outer capsid protein VP4, partial Protein is expressed from Yeast with N-terminal 6xHis-tagged. It contains 1-249aa. [Accession | A9Q1L0].
Uniprot No.A9Q1L0
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region1-249aa
SpeciesRotavirus X (isolate RVX/Human/Bangladesh/NADRV-B219/2002/GXP[X]) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-B219))
Tag InfoN-terminal 6xHis-tagged
Molecular weight30.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Forms the head of the spikes and mediates the recognition of specific host cell surface glycans. It is the viral hemagglutinin and an important target of neutralizing antibodies.

Biological Context

Subcellular Location: Virion; Host rough endoplasmic reticulum; Host cell membrane; Host endoplasmic reticulum-Golgi intermediate compartment

Product Specifications

Recombinant Rotavirus X Outer capsid protein VP4, partial is a recombinant protein from Rotavirus X (isolate RVX/Human/Bangladesh/NADRV-B219/2002/GXP[X]) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-B219)), expressed in Yeast, covering amino acids 1-249aa, with N-terminal 6xHis-tagged tag, molecular weight 30.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Forms the head of the spikes and mediates the recognition of specific host cell surface glycans. It is the viral hemagglutinin and an important target of neutralizing antibodies

Subcellular Location

Virion; Host rough endoplasmic reticulum; Host cell membrane; Host endoplasmic reticulum-Golgi intermediate compartment

Subunit

Homotrimer. The trimer is coiled-coil stabilized by its C-terminus, however, its N-terminus, known as antigen domain or 'body', seems to be flexible allowing it to self-associate either as a dimer or a trimer

Organism: Rotavirus X (isolate RVX/Human/Bangladesh/NADRV-B219/2002/GXP[X])  |  Synonyms: Hemagglutinin

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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