Recombinant Saccharomyces cerevisiae DNA-binding protein RAP1 (RAP1), partial, Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast), E.coli, Tag-Free, 28.7kDa, purity >85% as determined by SDS-PAGE.

Recombinant Saccharomyces cerevisiae DNA-binding protein RAP1 (RAP1), partial

Recombinant Saccharomyces cerevisiae DNA-binding protein RAP1 (RAP1), partial — Essential regulatory protein in yeast whose DNA-binding sites are found at three types of chromosomal elements: promoters, silencers, and telomeres. Purity >85%.

SKU: BCRECP-004233 Category:
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Product Specifications

Product SkuBCRECP-004233
Product DescriptionRecombinant Saccharomyces cerevisiae DNA-binding protein RAP1 (RAP1) Protein is expressed from E.coli with Tag-Free. It contains 353-598aa. [Accession | P11938].
Uniprot No.P11938
Gene NamesRAP1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region353-598aa
SpeciesSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Tag InfoTag-Free
Molecular weight28.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Essential regulatory protein in yeast whose DNA-binding sites are found at three types of chromosomal elements: promoters, silencers, and telomeres. RAP1 is also involved in the regulation of telomere structure, where its binding sites are found within the terminal poly[C(1-3)A] sequences. The opposite regulatory functions of RAP1 are not intrinsic to its binding sites but, instead, result from interactions with different factors at promoters and silencers.

Biological Context

Subcellular Location: Nucleus; Chromosome, telomere

Product Specifications

Recombinant Saccharomyces cerevisiae DNA-binding protein RAP1 (RAP1), partial is a recombinant protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast), expressed in E.coli, covering amino acids 353-598aa, with Tag-Free tag, molecular weight 28.7kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Essential regulatory protein in yeast whose DNA-binding sites are found at three types of chromosomal elements: promoters, silencers, and telomeres (PubMed:3315231, PubMed:8194531). RAP1 is also involved in the regulation of telomere structure, where its binding sites are found within the terminal poly[C(1-3)A] sequences (PubMed:3315231, PubMed:8194531). The opposite regulatory functions of RAP1 are not intrinsic to its binding sites but, instead, result from interactions with different factors at promoters and silencers (PubMed:7867933, PubMed:8508768). RAP1 mediates repression of the HM loci and telomeres by recruiting the SIR complex (PubMed:7867933). May also target the binding of RIF1 and RIF2 to silencers and telomeres (PubMed:7867933). Forms with GCR1 a transcriptional activation complex that is required for expression of glycolytic and ribosomal gene (PubMed:8508768)

Subcellular Location

Nucleus; Chromosome, telomere

Subunit

Interacts (via C-terminus) with RIF1 (PubMed:7867933). Interacts (via C-terminus) with SIR3 (PubMed:7867933). Interacts (via C-terminus) with SIR4 (PubMed:7867933). Interacts with a GCR1 homodimer (PubMed:8508768)

Gene: RAP1  |  Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)  |  Synonyms: Repressor/activator site-binding protein; SBF-E; TUF
Key Publications

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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