Recombinant Saccharomyces cerevisiae DNA-binding protein RAP1 (RAP1), partial, Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast), E.coli, Tag-Free, 28.7kDa, purity >85% as determined by SDS-PAGE.

Recombinant Saccharomyces cerevisiae DNA-binding protein RAP1 (RAP1), partial

Recombinant Saccharomyces cerevisiae DNA-binding protein RAP1 (RAP1), partial — Essential regulatory protein in yeast whose DNA-binding sites are found at three types of chromosomal elements: promoters, silencers, and telomeres. Purity >85%.

SKU: BCRECP-004233 Category:
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Product Specifications

Product SkuBCRECP-004233
Product DescriptionRecombinant Saccharomyces cerevisiae DNA-binding protein RAP1 (RAP1) Protein is expressed from E.coli with Tag-Free. It contains 353-598aa. [Accession | P11938].
Uniprot No.P11938
Gene NamesRAP1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region353-598aa
SpeciesSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Tag InfoTag-Free
Molecular weight28.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Essential regulatory protein in yeast whose DNA-binding sites are found at three types of chromosomal elements: promoters, silencers, and telomeres. RAP1 is also involved in the regulation of telomere structure, where its binding sites are found within the terminal poly[C(1-3)A] sequences. The opposite regulatory functions of RAP1 are not intrinsic to its binding sites but, instead, result from interactions with different factors at promoters and silencers.

Biological Context

Subcellular Location: Nucleus; Chromosome, telomere

Product Specifications

Recombinant Saccharomyces cerevisiae DNA-binding protein RAP1 (RAP1), partial is a recombinant protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast), expressed in E.coli, covering amino acids 353-598aa, with Tag-Free tag, molecular weight 28.7kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Essential regulatory protein in yeast whose DNA-binding sites are found at three types of chromosomal elements: promoters, silencers, and telomeres (PubMed:3315231, PubMed:8194531). RAP1 is also involved in the regulation of telomere structure, where its binding sites are found within the terminal poly[C(1-3)A] sequences (PubMed:3315231, PubMed:8194531). The opposite regulatory functions of RAP1 are not intrinsic to its binding sites but, instead, result from interactions with different factors at promoters and silencers (PubMed:7867933, PubMed:8508768). RAP1 mediates repression of the HM loci and telomeres by recruiting the SIR complex (PubMed:7867933). May also target the binding of RIF1 and RIF2 to silencers and telomeres (PubMed:7867933). Forms with GCR1 a transcriptional activation complex that is required for expression of glycolytic and ribosomal gene (PubMed:8508768)

Subcellular Location

Nucleus; Chromosome, telomere

Subunit

Interacts (via C-terminus) with RIF1 (PubMed:7867933). Interacts (via C-terminus) with SIR3 (PubMed:7867933). Interacts (via C-terminus) with SIR4 (PubMed:7867933). Interacts with a GCR1 homodimer (PubMed:8508768)

Gene: RAP1  |  Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)  |  Synonyms: Repressor/activator site-binding protein; SBF-E; TUF
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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