Recombinant Saccharomyces cerevisiae Nicotinamidase (PNC1), Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast), E.coli, Tag-Free, 25.0kDa, purity >90% as determined by SDS-PAGE.

Recombinant Saccharomyces cerevisiae Nicotinamidase (PNC1)

Recombinant Saccharomyces cerevisiae Nicotinamidase (PNC1) — Catalyzes the deamidation of nicotinamide, an early step in the NAD(+) salvage pathway. Purity >90%.

SKU: BCRECP-004125 Category:
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Product Specifications

Product SkuBCRECP-004125
Product DescriptionRecombinant Saccharomyces cerevisiae Nicotinamidase (PNC1) Protein is expressed from E.coli with Tag-Free. It contains 1-216aa. [Accession | P53184].
Uniprot No.P53184
Gene NamesPNC1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-216aa
SpeciesSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Tag InfoTag-Free
Molecular weight25.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

Catalyzes the deamidation of nicotinamide, an early step in the NAD(+) salvage pathway. Positively regulates SIR2-mediated silencing and longevity by preventing the accumulation of intracellular nicotinamide, an inhibitor of SIR2, during times of stress. Also acts on nicotinyl hydroxamate.

Biological Context

Subcellular Location: Cytoplasm; Nucleus; Peroxisome
Pathway: Cofactor biosynthesis; nicotinate biosynthesis; nicotinate from nicotinamide: step 1/1

Product Specifications

Recombinant Saccharomyces cerevisiae Nicotinamidase (PNC1) is a recombinant protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast), expressed in E.coli, covering amino acids 1-216aa, with Tag-Free tag, molecular weight 25.0kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Catalyzes the deamidation of nicotinamide, an early step in the NAD(+) salvage pathway. Positively regulates SIR2-mediated silencing and longevity by preventing the accumulation of intracellular nicotinamide, an inhibitor of SIR2, during times of stress. Also acts on nicotinyl hydroxamate

Subcellular Location

Cytoplasm; Nucleus; Peroxisome

Pathway

Cofactor biosynthesis; nicotinate biosynthesis; nicotinate from nicotinamide: step 1/1

Gene: PNC1  |  Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)  |  Synonyms: Nicotinamide deamidase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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