Recombinant Saccharomyces cerevisiae Nicotinamidase (PNC1), Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast), E.coli, Tag-Free, 25.0kDa, purity >90% as determined by SDS-PAGE.

Recombinant Saccharomyces cerevisiae Nicotinamidase (PNC1)

Recombinant Saccharomyces cerevisiae Nicotinamidase (PNC1) — Catalyzes the deamidation of nicotinamide, an early step in the NAD(+) salvage pathway. Purity >90%.

SKU: BCRECP-004125 Category:
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Product Specifications

Product SkuBCRECP-004125
Product DescriptionRecombinant Saccharomyces cerevisiae Nicotinamidase (PNC1) Protein is expressed from E.coli with Tag-Free. It contains 1-216aa. [Accession | P53184].
Uniprot No.P53184
Gene NamesPNC1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-216aa
SpeciesSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Tag InfoTag-Free
Molecular weight25.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

Catalyzes the deamidation of nicotinamide, an early step in the NAD(+) salvage pathway. Positively regulates SIR2-mediated silencing and longevity by preventing the accumulation of intracellular nicotinamide, an inhibitor of SIR2, during times of stress. Also acts on nicotinyl hydroxamate.

Biological Context

Subcellular Location: Cytoplasm; Nucleus; Peroxisome
Pathway: Cofactor biosynthesis; nicotinate biosynthesis; nicotinate from nicotinamide: step 1/1

Product Specifications

Recombinant Saccharomyces cerevisiae Nicotinamidase (PNC1) is a recombinant protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast), expressed in E.coli, covering amino acids 1-216aa, with Tag-Free tag, molecular weight 25.0kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

Catalyzes the deamidation of nicotinamide, an early step in the NAD(+) salvage pathway. Positively regulates SIR2-mediated silencing and longevity by preventing the accumulation of intracellular nicotinamide, an inhibitor of SIR2, during times of stress. Also acts on nicotinyl hydroxamate

Subcellular Location

Cytoplasm; Nucleus; Peroxisome

Pathway

Cofactor biosynthesis; nicotinate biosynthesis; nicotinate from nicotinamide: step 1/1

Gene: PNC1  |  Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)  |  Synonyms: Nicotinamide deamidase
Key Publications

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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