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Recombinant Saccharomyces cerevisiae Nucleoside diphosphate kinase (YNK1)

Recombinant Saccharomyces cerevisiae Nucleoside diphosphate kinase (YNK1) — Major role in the synthesis of nucleoside triphosphates other than ATP. Purity >85%.

SKU: BCRECP-004768 Categories: , ,

Product Specifications

Product SkuBCRECP-004768
Product DescriptionRecombinant Saccharomyces cerevisiae Nucleoside diphosphate kinase (YNK1) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-153aa. [Accession | P36010].
Uniprot No.P36010
Gene NamesYNK1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-153aa
SpeciesSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Tag InfoN-terminal 6xHis-tagged
Molecular weight21.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Required for repair of UV radiation- and etoposide-induced DNA damage.

Biological Context

Subcellular Location: Cytoplasm; Mitochondrion intermembrane space

Product Specifications

Recombinant Saccharomyces cerevisiae Nucleoside diphosphate kinase (YNK1) is a recombinant protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast), expressed in E.coli, covering amino acids 1-153aa, with N-terminal 6xHis-tagged tag, molecular weight 21.2kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Protein Biology

Function

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate (PubMed:5793714). Required for repair of UV radiation- and etoposide-induced DNA damage (PubMed:18983998)

Subcellular Location

Cytoplasm; Mitochondrion intermembrane space

Subunit

Homohexamer and homotetramer. Interacts with TOM40 preferentially in an unfolded, unphosphorylated form

Gene: YNK1  |  Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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