Recombinant Saccharomyces cerevisiae Phosphoglycerate mutase 1 (GPM1), Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast), Yeast, N-terminal 6xHis-tagged, 29.5kDa, purity >90% as determined by SDS-PAGE.

Recombinant Saccharomyces cerevisiae Phosphoglycerate mutase 1 (GPM1)

Recombinant Saccharomyces cerevisiae Phosphoglycerate mutase 1 (GPM1) — Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Purity >90%.

SKU: BCRECP-002905 Category:
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Product Specifications

Product SkuBCRECP-002905
Product DescriptionRecombinant Saccharomyces cerevisiae Phosphoglycerate mutase 1 (GPM1) Protein is expressed from Yeast with N-terminal 6xHis-tagged. It contains 2-247aa. [Accession | P00950].
Uniprot No.P00950
Gene NamesGPM1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region2-247aa
SpeciesSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Tag InfoN-terminal 6xHis-tagged
Molecular weight29.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research Areasothers

Function

Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase), but with a reduced activity.

Biological Context

Subcellular Location: Cytoplasm; Mitochondrion outer membrane (Peripheral membrane protein); Mitochondrion intermembrane space
Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 3/5

Product Specifications

Recombinant Saccharomyces cerevisiae Phosphoglycerate mutase 1 (GPM1) is a recombinant protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast), expressed in Yeast, covering amino acids 2-247aa, with N-terminal 6xHis-tagged tag, molecular weight 29.5kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase), but with a reduced activity

Subcellular Location

Cytoplasm; Mitochondrion outer membrane (Peripheral membrane protein); Mitochondrion intermembrane space

Subunit

Homotetramer: dimer of dimers

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 3/5

Gene: GPM1  |  Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)  |  Synonyms: BPG-dependent PGAM 1; MPGM 1; Phosphoglyceromutase 1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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