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Recombinant Sandfly fever sicilian virus Nucleoprotein (N)

Recombinant Sandfly fever sicilian virus Nucleoprotein (N) — Encapsidates the genomic RNA, protecting it from nucleases. Purity >85%.

SKU: BCRECP-003454 Categories: ,

Product Specifications

Product SkuBCRECP-003454
Product DescriptionRecombinant Sandfly fever sicilian virus Nucleoprotein (N) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-246aa. [Accession | P12793].
Uniprot No.P12793
Gene NamesN
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-246aa
SpeciesSandfly fever sicilian virus (SFS)
Tag InfoN-terminal 6xHis-tagged
Molecular weight32.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Encapsidates the genomic RNA, protecting it from nucleases. Displays high affinity for single-stranded nucleic acid.

Product Specifications

Recombinant Sandfly fever sicilian virus Nucleoprotein (N) — Encapsidates the genomic RNA, protecting it from nucleases. Purity >85%. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Protein Biology

Function

Encapsidates the genomic RNA, protecting it from nucleases. Displays high affinity for single-stranded nucleic acid. The encapsidated genomic RNA is termed the nucleocapsid (NC) or ribonucleoprotein. The ribonucleoprotein has a non-helical structure (By similarity). Serves as template for viral transcription and replication. After replication, the nucleocapsid is recruited to the host Golgi apparatus by glycoprotein Gn for packaging into virus particles (By similarity)

Subcellular Location

Virion; Host cytoplasm; Host nucleus; Host endoplasmic reticulum-Golgi intermediate compartment; Host Golgi apparatus

Subunit

Homodimer. Homohexamer; ring-shaped, necessary to form the nucleocapsid (By similarity). Homopentamers; opened pentamers in solution (By similarity). Binds to viral genomic RNA (By similarity). Interacts with glycoprotein Gn; this interaction allows packaging of nucleocapsids into virions (By similarity)

Gene: N  |  Organism: Sandfly fever sicilian virus  |  Synonyms: Nucleocapsid protein

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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