Recombinant Schizosaccharomyces pombe Glucan endo-1,3-alpha-glucosidase agn1 (agn1)

Recombinant Schizosaccharomyces pombe Glucan endo-1,3-alpha-glucosidase agn1 (agn1) — Has a role in cell separation where it is required for the degradation of the cell wall material surrounding the septum (the septum edging) which must be hydrolyzed before full separation of the da… Purity >85%.

SKU: BCRECP-002037 Category:

Product Specifications

Product SkuBCRECP-002037
Product DescriptionRecombinant Schizosaccharomyces pombe Glucan endo-1,3-alpha-glucosidase agn1 (agn1) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 21-424aa. [Accession | O13716].
Uniprot No.O13716
Gene Namesagn1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region21-424aa
SpeciesSchizosaccharoMyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight51.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Has a role in cell separation where it is required for the degradation of the cell wall material surrounding the septum (the septum edging) which must be hydrolyzed before full separation of the daughter cells can occur. Hydrolyzes 1,3-alpha-glucan predominantly into pentasaccharides.

Biological Context

Subcellular Location: Secreted; Secreted, cell wall

Product Specifications

Recombinant Schizosaccharomyces pombe Glucan endo-1,3-alpha-glucosidase agn1 (agn1) is a recombinant protein from SchizosaccharoMyces pombe (strain 972 / ATCC 24843) (Fission yeast), expressed in E.coli, covering amino acids 21-424aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 51.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Protein Biology

Function

Has a role in cell separation where it is required for the degradation of the cell wall material surrounding the septum (the septum edging) which must be hydrolyzed before full separation of the daughter cells can occur. Hydrolyzes 1,3-alpha-glucan predominantly into pentasaccharides

Subcellular Location

Secreted; Secreted, cell wall

Subunit

Monomer

Gene: agn1  |  Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843)  |  Synonyms: Endo-1,3-alpha-glucanase agn1

Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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