Recombinant Schizosaccharomyces pombe Glucan endo-1,3-alpha-glucosidase agn1 (agn1), SchizosaccharoMyces pombe (strain 972 / ATCC 24843) (Fission yeast), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 51.8kDa, purity >85% as determined by SDS-PAGE.

Recombinant Schizosaccharomyces pombe Glucan endo-1,3-alpha-glucosidase agn1 (agn1)

Recombinant Schizosaccharomyces pombe Glucan endo-1,3-alpha-glucosidase agn1 (agn1) — Has a role in cell separation where it is required for the degradation of the cell wall material surrounding the septum (the septum edging) which must be hydrolyzed before full separation of the da… Purity >85%.

SKU: BCRECP-002037 Category:
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Product Specifications

Product SkuBCRECP-002037
Product DescriptionRecombinant Schizosaccharomyces pombe Glucan endo-1,3-alpha-glucosidase agn1 (agn1) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 21-424aa. [Accession | O13716].
Uniprot No.O13716
Gene Namesagn1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region21-424aa
SpeciesSchizosaccharoMyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight51.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Has a role in cell separation where it is required for the degradation of the cell wall material surrounding the septum (the septum edging) which must be hydrolyzed before full separation of the daughter cells can occur. Hydrolyzes 1,3-alpha-glucan predominantly into pentasaccharides.

Biological Context

Subcellular Location: Secreted; Secreted, cell wall

Product Specifications

Recombinant Schizosaccharomyces pombe Glucan endo-1,3-alpha-glucosidase agn1 (agn1) is a recombinant protein from SchizosaccharoMyces pombe (strain 972 / ATCC 24843) (Fission yeast), expressed in E.coli, covering amino acids 21-424aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 51.8kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Has a role in cell separation where it is required for the degradation of the cell wall material surrounding the septum (the septum edging) which must be hydrolyzed before full separation of the daughter cells can occur. Hydrolyzes 1,3-alpha-glucan predominantly into pentasaccharides

Subcellular Location

Secreted; Secreted, cell wall

Subunit

Monomer

Gene: agn1  |  Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843)  |  Synonyms: Endo-1,3-alpha-glucanase agn1

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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