Recombinant Semliki forest virus Polyprotein P1234, partial, Semliki forest virus (SFV), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 33.2kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Semliki forest virus Polyprotein P1234, partial

Recombinant Semliki forest virus Polyprotein P1234, partial — RNA dependent RNA polymerase. Purity >85%.

SKU: BCRECP-003691 Categories: ,
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Product Specifications

Product SkuBCRECP-003691
Product DescriptionRecombinant Semliki forest virus Polyprotein P1234, partial Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 29-260aa. [Accession | P08411].
Uniprot No.P08411
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region29-260aa
SpeciesSemliki forest virus (SFV)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight33.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

RNA dependent RNA polymerase. Replicates genomic and antigenomic RNA by recognizing replications specific signals. The early replication complex formed by the polyprotein P123 and nsP4 synthesizes minus-strand RNAs.

Biological Context

Subcellular Location: Host cytoplasmic vesicle membrane (Peripheral membrane protein)

Product Specifications

Recombinant Semliki forest virus Polyprotein P1234, partial is a recombinant protein from Semliki forest virus (SFV), expressed in E.coli, covering amino acids 29-260aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 33.2kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

RNA dependent RNA polymerase (By similarity). Replicates genomic and antigenomic RNA by recognizing replications specific signals. The early replication complex formed by the polyprotein P123 and nsP4 synthesizes minus-strand RNAs (By similarity). The late replication complex composed of fully processed nsP1-nsP4 is responsible for the production of genomic and subgenomic plus-strand RNAs (By similarity)

Subcellular Location

Host cytoplasmic vesicle membrane (Peripheral membrane protein)

Subunit

Interacts with RNA-directed RNA polymerase nsP4 (By similarity). Interacts with mRNA-capping enzyme nsP1 (By similarity). Interacts with KPNA1/karyopherin-alpha1; this interaction probably allows the active transport of protease nsP2 into the host nucleus (By similarity)

Organism: Semliki forest virus  |  Synonyms: Non-structural polyprotein
Key Publications

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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