Recombinant Severe acute respiratory syndrome coronavirus 2 Nucleoprotein (N) (D63G,R203M,D377Y) (Active), Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2), E.coli, N-terminal 6xHis-tagged, 49.1 kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Severe acute respiratory syndrome coronavirus 2 Nucleoprotein (N) (D63G,R203M,D377Y) (Active)

Recombinant Severe acute respiratory syndrome coronavirus 2 Nucleoprotein (N) (D63G,R203M,D377Y) (Active) — May induce inflammasome responses in cultured cells and mice. Purity >85%.

SKU: BCACP-000141 Categories: , , ,
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Product SKUBCACP-000141
Product DescriptionRecombinant SARS-CoV-2 Nucleoprotein (N) (D63G, R203M, D377Y) protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-419aa(D63G,R203M,D377Y). [Accession | P0DTC9].
Uniprot No.P0DTC9
Gene NamesN
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-419aa(D63G,R203M,D377Y)
SpeciesSevere acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
Tag InfoN-terminal 6xHis-tagged
Molecular weight49.1 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized HPV16 E7 at 10 μg/mL can bind Biotinylated MYC（CSB-EP015270HU-B）, the EC50 is 268.1-354.3 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesN; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N

Function

May induce inflammasome responses in cultured cells and mice. Acts by interacting with host NLRP3 to facilitate inflammasome assembly, which induces cytokine release that may play a role in COVID lung injury. Disrupts the association betwen NLRP3 and ABHD8, enhancing NLRP3 stability and leading to increased inflammasome activation.

Biological Context

Subcellular Location: Virion; Host cytoplasm; Secreted; Host extracellular space

Product Specifications

Recombinant Severe acute respiratory syndrome coronavirus 2 Nucleoprotein (N) (D63G,R203M,D377Y) (Active) is a recombinant protein from Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2), expressed in E.coli, covering amino acids 1-419aa(D63G,R203M,D377Y), with N-terminal 6xHis-tagged tag, molecular weight 49.1 kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

May induce inflammasome responses in cultured cells and mice. Acts by interacting with host NLRP3 to facilitate inflammasome assembly, which induces cytokine release that may play a role in COVID lung injury. Disrupts the association betwen NLRP3 and ABHD8, enhancing NLRP3 stability and leading to increased inflammasome activation (PubMed:39225180)

Subcellular Location

Virion; Host cytoplasm; Secreted; Host extracellular space

Subunit

Homodimer, homotetramer, homooligomer with RNA (PubMed:32654247, PubMed:38172120). Both monomeric and oligomeric forms interact with RNA. Interacts with protein M (By similarity). Interacts with protein E (By similarity). Interacts with NSP3; this interaction serves to tether the genome to the newly translated replicase-transcriptase complex at a very early stage of infection (PubMed:35044811). Interacts with host NLRP3 and ABHD8; these interactions disrupt the association between NLRP3 and ABHD8, increasing NLRP3 stability, ultimately leading to increased inflammasome activation (PubMed:34341353, PubMed:39225180). Interacts with host G3BP1; the interaction is enhanced by host HDAC6 which deacetylates the viral N protein and promotes N protein association with G3BP1, disrupting stress granule formation and facilitating viral replication (PubMed:39135075)

Gene: N  |  Organism: Severe acute respiratory syndrome coronavirus 2  |  Synonyms: Nucleocapsid protein
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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