Recombinant Severe acute respiratory syndrome coronavirus 2 Replicase polyprotein 1ab (rep), partial, Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2), E.coli, N-terminal 10xHis-tagged, 41.7kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Severe acute respiratory syndrome coronavirus 2 Replicase polyprotein 1ab (rep), partial

Recombinant Severe acute respiratory syndrome coronavirus 2 Replicase polyprotein 1ab (rep), partial — Methyltransferase that mediates mRNA cap 2′-O-ribose methylation to the 5′-cap structure of viral mRNAs. Purity >85%.

SKU: BCRECP-004256 Categories: , , ,
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Product Specifications

Product SkuBCRECP-004256
Product DescriptionRecombinant Severe acute respiratory syndrome coronavirus 2 Replicase polyprotein 1ab (rep) Protein is expressed from E.coli with N-terminal 10xHis-tagged. It contains 1564-1878aa. [Accession | P0DTD1].
Uniprot No.P0DTD1
Gene Namesrep
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1564-1878aa
SpeciesSevere acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
Tag InfoN-terminal 10xHis-tagged
Molecular weight41.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Methyltransferase that mediates mRNA cap 2'-O-ribose methylation to the 5'-cap structure of viral mRNAs. N7-methyl guanosine cap is a prerequisite for binding of nsp16. Therefore, it plays an essential role in cap methylation of viral mRNAs, which is essential to evade the immune system, especially when restricted by human IFIT1 and IFIT3.

Biological Context

Subcellular Location: Host nucleus; Host cytoplasm

Product Specifications

Recombinant Severe acute respiratory syndrome coronavirus 2 Replicase polyprotein 1ab (rep), partial is a recombinant protein from Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2), expressed in E.coli, covering amino acids 1564-1878aa, with N-terminal 10xHis-tagged tag, molecular weight 41.7kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Methyltransferase that mediates mRNA cap 2'-O-ribose methylation to the 5'-cap structure of viral mRNAs (PubMed:35944563). N7-methyl guanosine cap is a prerequisite for binding of nsp16 (PubMed:35944563). Therefore, it plays an essential role in cap methylation of viral mRNAs, which is essential to evade the immune system, especially when restricted by human IFIT1 and IFIT3 (PubMed:35944563, PubMed:36722972, PubMed:36285486). May disrupt host mRNA splicing in nucleus by interacting with pre-mRNA Recognition Domains of the U1 and U2 snRNAs (PubMed:33080218)

Subcellular Location

Host nucleus; Host cytoplasm

Subunit

Interacts with nsp10

Gene: rep  |  Organism: Severe acute respiratory syndrome coronavirus 2  |  Synonyms: ORF1ab polyprotein
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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