Recombinant Severe acute respiratory syndrome coronavirus 2 Replicase polyprotein 1ab (rep), partial, Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2), E.coli, N-terminal 10xHis-tagged, 41.7kDa, purity >85% as determined by SDS-PAGE.
, ,

Recombinant Severe acute respiratory syndrome coronavirus 2 Replicase polyprotein 1ab (rep), partial

Recombinant Severe acute respiratory syndrome coronavirus 2 Replicase polyprotein 1ab (rep), partial — Methyltransferase that mediates mRNA cap 2′-O-ribose methylation to the 5′-cap structure of viral mRNAs. Purity >85%.

SKU: BCRECP-004256 Categories: , ,
Online Inquiry →

Product Specifications

Product SkuBCRECP-004256
Product DescriptionRecombinant Severe acute respiratory syndrome coronavirus 2 Replicase polyprotein 1ab (rep) Protein is expressed from E.coli with N-terminal 10xHis-tagged. It contains 1564-1878aa. [Accession | P0DTD1].
Uniprot No.P0DTD1
Gene Namesrep
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1564-1878aa
SpeciesSevere acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
Tag InfoN-terminal 10xHis-tagged
Molecular weight41.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Methyltransferase that mediates mRNA cap 2'-O-ribose methylation to the 5'-cap structure of viral mRNAs. N7-methyl guanosine cap is a prerequisite for binding of nsp16. Therefore, it plays an essential role in cap methylation of viral mRNAs, which is essential to evade the immune system, especially when restricted by human IFIT1 and IFIT3.

Biological Context

Subcellular Location: Host nucleus; Host cytoplasm

Product Specifications

Recombinant Severe acute respiratory syndrome coronavirus 2 Replicase polyprotein 1ab (rep), partial is a recombinant protein from Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2), expressed in E.coli, covering amino acids 1564-1878aa, with N-terminal 10xHis-tagged tag, molecular weight 41.7kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

Methyltransferase that mediates mRNA cap 2'-O-ribose methylation to the 5'-cap structure of viral mRNAs (PubMed:35944563). N7-methyl guanosine cap is a prerequisite for binding of nsp16 (PubMed:35944563). Therefore, it plays an essential role in cap methylation of viral mRNAs, which is essential to evade the immune system, especially when restricted by human IFIT1 and IFIT3 (PubMed:35944563, PubMed:36722972, PubMed:36285486). May disrupt host mRNA splicing in nucleus by interacting with pre-mRNA Recognition Domains of the U1 and U2 snRNAs (PubMed:33080218)

Subcellular Location

Host nucleus; Host cytoplasm

Subunit

Interacts with nsp10

Gene: rep  |  Organism: Severe acute respiratory syndrome coronavirus 2  |  Synonyms: ORF1ab polyprotein
Key Publications

Explore closely related product collections, technology platforms, and custom production resources.

Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

Shopping Cart
Scroll to Top