| Product SKU | BCACP-000059 |
| Product Description | Recombinant SARS-CoV-2 Spike glycoprotein (S) protein is expressed from Mammalian cell with C-terminal 6xHis-mFc-tagged. It contains 319-541aa. [Accession | P0DTC2]. |
| Uniprot No. | P0DTC2 |
| Gene Names | S |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Expression System | Mammalian cell |
| Expression Region | 319-541aa |
| Species | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2) |
| Tag Info | C-terminal 6xHis-mFc-tagged |
| Molecular weight | 51.1 kDa |
| Activity | Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind SARS-CoV-2-S Antibody , the EC50 of SARS-CoV-2-S1-RBD protein is 19.60-39.42 ng/ml.;Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 5 μg/ml can bind human ACE2 , the EC50 of SARS-CoV-2-S1-RBD protein is 31.80 - 44.69 ng/ml.;Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind SARS-CoV-2-S Antibody , the EC50 of SARS-CoV-2-S1-RBD protein is 13.48-19.50 ng/ml.;SARS-CoV-2 Spike protein RBD his/sumostar tag captured on COOH chip can bind Human ACE2 protein Fc tag with an affinity constant of 100 nM as detected by LSPR Assay.;SARS-CoV-2 Spike protein RBD His/Sumostar Tag captured on COOH chip can bind SARS-CoV-2 Spike RBD Nanobody with an affinity constant of 28.2nM as detected by LSPR Assay. |
| Buffer | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Alternative Names | S; 2; Spike glycoprotein; S glycoprotein; E2; Peplomer protein) |
SDS-PAGE: Single band at expected molecular weight confirming purity.
ELISA: Suitable as coating antigen or detection standard.
Western Blot: Compatible with standard Western Blot protocols.
Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.
Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.
Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.
Shelf Life: 12 months from date of receipt when stored as recommended.
Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.
