Recombinant Severe acute respiratory syndrome coronavirus 2 Spike glycoprotein (S), partial,Biotinylated (Active), Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2), Mammalian cell, C-terminal mFC-Avi-tagged, 54.1 kDa, purity >90% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.
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Recombinant Severe acute respiratory syndrome coronavirus 2 Spike glycoprotein (S), partial,Biotinylated (Active)

Recombinant Severe acute respiratory syndrome coronavirus 2 Spike glycoprotein (S), partial,Biotinylated (Active) — Subunit of the fusion protein that is processed upon entry into the host cell. Purity >90%.

SKU: BCACP-000058 Categories: , , , ,
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Product SKUBCACP-000058
Product DescriptionRecombinant SARS-CoV-2 Spike glycoprotein (S), Biotinylated protein is expressed from Mammalian cell with C-terminal mFC-Avi-tagged. It contains 319-541aa. [Accession | P0DTC2].
Uniprot No.P0DTC2
Gene NamesS
PurityGreater than 90% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.
Expression SystemMammalian cell
Expression Region319-541aa
SpeciesSevere acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
Tag InfoC-terminal mFC-Avi-tagged
Molecular weight54.1 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind SARS-CoV-2-S Antibody , the EC50 of SARS-CoV-2-S1-RBD protein is 27.96 - 33.35 ng/ml.;Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind SARS-CoV-2-S Antibody , the EC50 of SARS-CoV-2-S1-RBD protein is 13.96 -16.62 ng/ml.;Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 5 μg/ml can bind human ACE2 , the EC50 is 2.785-9.139 ng/ml.;SARS-CoV-2 Spike protein RBD his/myc tag captured on COOH chip can bind Human ACE2 protein Fc tag with an affinity constant of 13.8 nM as detected by LSPR Assay.;Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind Biotinylated human ACE2 , the EC50 is 4.599-8.322 ng/ml
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesS; 2; Spike glycoprotein; S glycoprotein; E2; Peplomer protein)

Function

Subunit of the fusion protein that is processed upon entry into the host cell. Mediates fusion of the virion and cellular membranes by functioning as a class I viral fusion protein. Contains a viral fusion peptide that is unmasked after S2 cleavage.

Biological Context

Subcellular Location: Virion membrane (Single-pass type I membrane protein); Host endoplasmic reticulum-Golgi intermediate compartment membrane (Single-pass type I membrane protein); Host cell membrane (Single-pass type I membrane protein)

Product Specifications

Recombinant Severe acute respiratory syndrome coronavirus 2 Spike glycoprotein (S), partial,Biotinylated (Active) is a recombinant protein from Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2), expressed in Mammalian cell, covering amino acids 319-541aa, with C-terminal mFC-Avi-tagged tag, molecular weight 54.1 kDa, purity Greater than 90% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind SARS-CoV-2-S Antibody , the EC50 of SARS-CoV-2-S1-RBD protein is 27.96 - 33.35 ng/ml.;Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind SARS-CoV-2-S Antibody , the EC50 of SARS-CoV-2-S1-RBD protein is 13.96 -16.62 ng/ml.;Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 5 μg/ml can bind human ACE2 , the EC50 is 2.785-9.139 ng/ml.;SARS-CoV-2 Spike protein RBD his/myc tag captured on COOH chip can bind Human ACE2 protein Fc tag with an affinity constant of 13.8 nM as detected by LSPR Assay.;Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind Biotinylated human ACE2 , the EC50 is 4.599-8.322 ng/ml

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Subunit of the fusion protein that is processed upon entry into the host cell. Mediates fusion of the virion and cellular membranes by functioning as a class I viral fusion protein. Contains a viral fusion peptide that is unmasked after S2 cleavage. This cleavage can occur at the cell membrane by host TMPRSS2 or during endocytosis by host CSTL (PubMed:32703818, PubMed:34159616). In either case, this triggers an extensive and irreversible conformational change that leads to fusion of the viral envelope with the cellular cytoplasmic membrane, releasing viral genomic RNA into the host cell cytoplasm (PubMed:34561887). Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During fusion of the viral and target cell membranes, the coiled coil regions (heptad repeats) adopt a trimer-of-hairpins structure and position the fusion peptide in close proximity to the C-terminal region of the ectodomain. Formation of this structure appears to promote apposition and subsequent fusion of viral and target cell membranes

Subcellular Location

Virion membrane (Single-pass type I membrane protein); Host endoplasmic reticulum-Golgi intermediate compartment membrane (Single-pass type I membrane protein); Host cell membrane (Single-pass type I membrane protein)

Subunit

Binds to host ACE2 (PubMed:32075877, PubMed:32132184, PubMed:32155444, PubMed:32221306, PubMed:32225175, PubMed:32225176, PubMed:33607086). RBD also interacts with the N-linked glycan on 'Asn-90' of ACE2 (PubMed:33607086). Cleavage of S generates a polybasic C-terminal sequence on S1 that binds to host Neuropilin-1 (NRP1) and Neuropilin-2 (NRP2) receptors (PubMed:33082293, PubMed:33082294). Interacts with host integrin alpha-5/beta-1 (ITGA5:ITGB1) and with ACE2 in complex with integrin alpha-5/beta-1 (ITGA5:ITGB1) (PubMed:33102950). May interact via cytoplasmic c-terminus with M protein (PubMed:33229438). May interact (via N-terminus) with host bilirubin and biliverdin, thereby preventing antibody binding to the SARS-CoV-2 spike NTD via an allosteric mechanism (PubMed:33888467). May interact with host LRRC15, thereby allowing attachment to host cells (PubMed:36735681)

Gene: S  |  Organism: Severe acute respiratory syndrome coronavirus 2  |  Synonyms: E2; Peplomer protein
Key Publications

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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

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Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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