Recombinant Severe acute respiratory syndrome coronavirus Spike glycoprotein (S), partial (Active), Human SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus), Mammalian cell, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 30.0 kDa, purity >95% as determined by SDS-PAGE.
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Recombinant Severe acute respiratory syndrome coronavirus Spike glycoprotein (S), partial (Active)

Recombinant Severe acute respiratory syndrome coronavirus Spike glycoprotein (S), partial (Active) — recombinant protein from Human SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus), expressed in Mammalian cell. Purity >95%.

SKU: BCACP-000033 Categories: , ,
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Product SKUBCACP-000033
Product DescriptionRecombinant Severe acute respiratory syndrome coronavirus Spike glycoprotein (S) protein is expressed from Mammalian cell with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 306-527aa. [Accession | P59594].
Uniprot No.P59594
Gene NamesS
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region306-527aa
SpeciesHuman SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight30.0 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized SARS-CoV S-RBD at 2 μg/ml can bind Paguma larvata ACE2 , the EC50 is 5.056-7.559 ng/ml.;Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV S-RBD at 5 μg/ml can bind human ACE2 , the EC50 is 7.941-10.49 ng/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative Names(S glycoprotein)(E2)(Peplomer protein)(Spike protein S1)(Spike protein S2)

Function

Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.

Biological Context

Subcellular Location: Virion membrane (Single-pass type I membrane protein); Host endoplasmic reticulum-Golgi intermediate compartment membrane (Single-pass type I membrane protein); Host cell membrane (Single-pass type I membrane protein)

Product Specifications

Recombinant Severe acute respiratory syndrome coronavirus Spike glycoprotein (S), partial (Active) is a recombinant protein from Human SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus), expressed in Mammalian cell, covering amino acids 306-527aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 30.0 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV S-RBD at 2 μg/ml can bind Paguma larvata ACE2 , the EC50 is 5.056-7.559 ng/ml.;Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV S-RBD at 5 μg/ml can bind human ACE2 , the EC50 is 7.941-10.49 ng/ml.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis

Subcellular Location

Virion membrane (Single-pass type I membrane protein); Host endoplasmic reticulum-Golgi intermediate compartment membrane (Single-pass type I membrane protein); Host cell membrane (Single-pass type I membrane protein)

Subunit

Homotrimer; each monomer consists of a S1 and a S2 subunit. The resulting peplomers protrude from the virus surface as spikes (By similarity). Binds to human and palm civet ACE2 and human CLEC4M/DC-SIGNR. Interacts with the accessory proteins 3a and 7a

Gene: S  |  Organism: Severe acute respiratory syndrome coronavirus  |  Synonyms: E2; Peplomer protein
Key Publications

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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