Recombinant Shigella flexneri Cysteine protease-like VirA (virA), Shigella flexneri, E.coli, C-terminal 6xHis-tagged, 51.6kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Shigella flexneri Cysteine protease-like VirA (virA)

Recombinant Shigella flexneri Cysteine protease-like VirA (virA) — Alpha-tubulin-specific protease that is required for entry into epithelial cells and for subsequent intra- and intercellular spreading. Purity >90%.

SKU: BCRECP-004726 Categories: , ,
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Product Specifications

Product SkuBCRECP-004726
Product DescriptionRecombinant Shigella flexneri Cysteine protease-like VirA (virA) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 1-400aa. [Accession | Q7BU69].
Uniprot No.Q7BU69
Gene NamesvirA
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-400aa
SpeciesShigella flexneri
Tag InfoC-terminal 6xHis-tagged
Molecular weight51.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Alpha-tubulin-specific protease that is required for entry into epithelial cells and for subsequent intra- and intercellular spreading. Contributes to bacterial entry into epithelial cells by inducing microtubule (MT) destabilization and the formation of membrane ruffles. The membrane ruffling evoked by VirA results from the activation of host rac1, which is associated with the destruction of MT networks.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Shigella flexneri Cysteine protease-like VirA (virA) is a recombinant protein from Shigella flexneri, expressed in E.coli, covering amino acids 1-400aa, with C-terminal 6xHis-tagged tag, molecular weight 51.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Alpha-tubulin-specific protease that is required for entry into epithelial cells and for subsequent intra- and intercellular spreading. Contributes to bacterial entry into epithelial cells by inducing microtubule (MT) destabilization and the formation of membrane ruffles. The membrane ruffling evoked by VirA results from the activation of host rac1, which is associated with the destruction of MT networks. Creates a tunnel inside the host cell cytoplasm by breaking down the microtubule infrastructure. This facilitates the bacterium's movement through the cytoplasm and also helps other bacteria move faster during the invasion of the eukaryotic cell. Is absolutely required for virulence

Subcellular Location

Secreted

Subunit

Monomer. Interacts specifically with alpha tubulin, a major component of microtubule

Gene: virA  |  Organism: Shigella flexneri  |  Synonyms: Effector protein VirA
Key Publications

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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