Recombinant Shigella flexneri Cysteine protease-like VirA (virA), Shigella flexneri, E.coli, C-terminal 6xHis-tagged, 51.6kDa, purity >90% as determined by SDS-PAGE.
, ,

Recombinant Shigella flexneri Cysteine protease-like VirA (virA)

Recombinant Shigella flexneri Cysteine protease-like VirA (virA) — Alpha-tubulin-specific protease that is required for entry into epithelial cells and for subsequent intra- and intercellular spreading. Purity >90%.

SKU: BCRECP-004726 Categories: , ,
Online Inquiry →

Product Specifications

Product SkuBCRECP-004726
Product DescriptionRecombinant Shigella flexneri Cysteine protease-like VirA (virA) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 1-400aa. [Accession | Q7BU69].
Uniprot No.Q7BU69
Gene NamesvirA
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-400aa
SpeciesShigella flexneri
Tag InfoC-terminal 6xHis-tagged
Molecular weight51.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Alpha-tubulin-specific protease that is required for entry into epithelial cells and for subsequent intra- and intercellular spreading. Contributes to bacterial entry into epithelial cells by inducing microtubule (MT) destabilization and the formation of membrane ruffles. The membrane ruffling evoked by VirA results from the activation of host rac1, which is associated with the destruction of MT networks.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Shigella flexneri Cysteine protease-like VirA (virA) is a recombinant protein from Shigella flexneri, expressed in E.coli, covering amino acids 1-400aa, with C-terminal 6xHis-tagged tag, molecular weight 51.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Alpha-tubulin-specific protease that is required for entry into epithelial cells and for subsequent intra- and intercellular spreading. Contributes to bacterial entry into epithelial cells by inducing microtubule (MT) destabilization and the formation of membrane ruffles. The membrane ruffling evoked by VirA results from the activation of host rac1, which is associated with the destruction of MT networks. Creates a tunnel inside the host cell cytoplasm by breaking down the microtubule infrastructure. This facilitates the bacterium's movement through the cytoplasm and also helps other bacteria move faster during the invasion of the eukaryotic cell. Is absolutely required for virulence

Subcellular Location

Secreted

Subunit

Monomer. Interacts specifically with alpha tubulin, a major component of microtubule

Gene: virA  |  Organism: Shigella flexneri  |  Synonyms: Effector protein VirA
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top