Recombinant Shigella flexneri Type 3 secretion system translocon protein SctB (sctB), Shigella flexneri, E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 46.2kDa, purity >85% as determined by SDS-PAGE.

Recombinant Shigella flexneri Type 3 secretion system translocon protein SctB (sctB)

Recombinant Shigella flexneri Type 3 secretion system translocon protein SctB (sctB) — Required for efficient dissemination. Purity >85%.

SKU: BCRECP-004385 Category:
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Product Specifications

Product SkuBCRECP-004385
Product DescriptionRecombinant Shigella flexneri Type 3 secretion system translocon protein SctB (sctB) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-363aa. [Accession | P18012].
Uniprot No.P18012
Gene NamessctB
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-363aa
SpeciesShigella flexneri
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight46.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Required for efficient dissemination. Necessary for lysis of the two cellular membranes that surround bacteria in protrusions during cell-to-cell spread. Contribute to actin nucleation in vitro, which may be a necessary step in Shigella invasion.

Biological Context

Subcellular Location: Secreted; Host membrane (Single-pass membrane protein)

Product Specifications

Recombinant Shigella flexneri Type 3 secretion system translocon protein SctB (sctB) is a recombinant protein from Shigella flexneri, expressed in E.coli, covering amino acids 1-363aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 46.2kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

Required for efficient dissemination (PubMed:11207551). Necessary for lysis of the two cellular membranes that surround bacteria in protrusions during cell-to-cell spread (PubMed:11207551). Contribute to actin nucleation in vitro, which may be a necessary step in Shigella invasion (PubMed:18656530)

Subcellular Location

Secreted; Host membrane (Single-pass membrane protein)

Subunit

The core secretion machinery of the T3SS is composed of approximately 20 different proteins, including cytoplasmic components, a base, an export apparatus and a needle (PubMed:30107569). This subunit is involved in the formation of a pore, called the translocon, in host membrane (PubMed:10545510, PubMed:17367391). Interacts with IpaB/SctE (PubMed:10545510, PubMed:17367391, PubMed:7954817). Interacts with the molecular chaperone IpgC, which prevents premature association with IpaB/SctE within the cytoplasm of Shigella cells (PubMed:11207551, PubMed:20937829, PubMed:7954817). Does not interact with CDC42 or RAC1 GTPases in vitro (PubMed:18656530)

Gene: sctB  |  Organism: Shigella flexneri  |  Synonyms: 42 kDa antigen; Invasin IpaC; Invasion plasmid antigen C
Key Publications

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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