Recombinant Shigella flexneri Type 3 secretion system translocon protein SctE (sctE), partial, Shigella flexneri, E.coli, N-terminal 6xHis-tagged, purity >>85% (SDS-PAGE)

Recombinant Shigella flexneri Type 3 secretion system translocon protein SctE (sctE), partial

Recombinant Shigella flexneri Type 3 secretion system translocon protein SctE (sctE), partial — Required for efficient dissemination. Purity >85% (SDS-PAGE).

SKU: BCRECP-004386 Category:
Online Inquiry →

Product Specifications

Product SkuBCRECP-004386
Product DescriptionRecombinant Shigella flexneri Type 3 secretion system translocon protein SctE (sctE) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-312aa. [Accession | P18011].
Uniprot No.P18011
Gene NamessctE
Purity>85% (SDS-PAGE)
Expression SystemE.coli
Expression Region1-312aa
SpeciesShigella flexneri
Tag InfoN-terminal 6xHis-tagged
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCell Biology

Function

Required for efficient dissemination. Necessary for lysis of the two cellular membranes that surround bacteria in protrusions during cell-to-cell spread. Is sufficient to induce macrophage apoptosis through activation of the interleukin-1 beta converting enzyme (ICE) in infected macrophages.

Biological Context

Subcellular Location: Secreted; Host membrane (Multi-pass membrane protein); Host cell; Host nucleus

Product Specifications

Recombinant Shigella flexneri Type 3 secretion system translocon protein SctE (sctE), partial is a recombinant protein from Shigella flexneri, expressed in E.coli, covering amino acids 1-312aa, with N-terminal 6xHis-tagged tag, purity >85% (SDS-PAGE). Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

Required for efficient dissemination (PubMed:11207551). Necessary for lysis of the two cellular membranes that surround bacteria in protrusions during cell-to-cell spread (PubMed:11207551). Is sufficient to induce macrophage apoptosis through activation of the interleukin-1 beta converting enzyme (ICE) in infected macrophages (PubMed:8670890, PubMed:9009343). In epithelial cells, causes cell-cycle arrest by targeting host MAD2L2, an anaphase-promoting complex/cyclosome (APC) inhibitor (PubMed:17719540)

Subcellular Location

Secreted; Host membrane (Multi-pass membrane protein); Host cell; Host nucleus

Subunit

The core secretion machinery of the T3SS is composed of approximately 20 different proteins, including cytoplasmic components, a base, an export apparatus and a needle (PubMed:30107569). This subunit is involved in the formation of a pore, called the translocon, in host membrane (PubMed:10545510, PubMed:17367391, PubMed:34809452). Interacts with IpaC/SctB (PubMed:10545510, PubMed:17367391, PubMed:7954817). Interacts with the needle tip protein IpaD/SctA (PubMed:17367391, PubMed:24236510). Interacts with the molecular chaperone IpgC, which prevents premature association with IpaC/SctB within the cytoplasm of Shigella cells and protects IpaB/SctE from proteolysis (PubMed:11207551, PubMed:19478065, PubMed:20937829, PubMed:7954817). Interacts with the host protein ICE in the cytoplasm of infected macrophages (PubMed:8670890). Interacts with human MAD2L2 in the G2/M phase of the cell cycle (PubMed:17719540, PubMed:31484720)

Gene: sctE  |  Organism: Shigella flexneri  |  Synonyms: 62 kDa antigen; Invasin IpaB
Key Publications

Explore closely related product collections, technology platforms, and custom production resources.

Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

Shopping Cart
Scroll to Top