Recombinant Shigella flexneri Type 3 secretion system translocon protein SctE (sctE), partial, Shigella flexneri, E.coli, N-terminal 6xHis-tagged, purity >>85% (SDS-PAGE)

Recombinant Shigella flexneri Type 3 secretion system translocon protein SctE (sctE), partial

Recombinant Shigella flexneri Type 3 secretion system translocon protein SctE (sctE), partial — Required for efficient dissemination. Purity >85% (SDS-PAGE).

SKU: BCRECP-004386 Category:
Online Inquiry →

Product Specifications

Product SkuBCRECP-004386
Product DescriptionRecombinant Shigella flexneri Type 3 secretion system translocon protein SctE (sctE) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-312aa. [Accession | P18011].
Uniprot No.P18011
Gene NamessctE
Purity>85% (SDS-PAGE)
Expression SystemE.coli
Expression Region1-312aa
SpeciesShigella flexneri
Tag InfoN-terminal 6xHis-tagged
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCell Biology

Function

Required for efficient dissemination. Necessary for lysis of the two cellular membranes that surround bacteria in protrusions during cell-to-cell spread. Is sufficient to induce macrophage apoptosis through activation of the interleukin-1 beta converting enzyme (ICE) in infected macrophages.

Biological Context

Subcellular Location: Secreted; Host membrane (Multi-pass membrane protein); Host cell; Host nucleus

Product Specifications

Recombinant Shigella flexneri Type 3 secretion system translocon protein SctE (sctE), partial is a recombinant protein from Shigella flexneri, expressed in E.coli, covering amino acids 1-312aa, with N-terminal 6xHis-tagged tag, purity >85% (SDS-PAGE). Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Required for efficient dissemination (PubMed:11207551). Necessary for lysis of the two cellular membranes that surround bacteria in protrusions during cell-to-cell spread (PubMed:11207551). Is sufficient to induce macrophage apoptosis through activation of the interleukin-1 beta converting enzyme (ICE) in infected macrophages (PubMed:8670890, PubMed:9009343). In epithelial cells, causes cell-cycle arrest by targeting host MAD2L2, an anaphase-promoting complex/cyclosome (APC) inhibitor (PubMed:17719540)

Subcellular Location

Secreted; Host membrane (Multi-pass membrane protein); Host cell; Host nucleus

Subunit

The core secretion machinery of the T3SS is composed of approximately 20 different proteins, including cytoplasmic components, a base, an export apparatus and a needle (PubMed:30107569). This subunit is involved in the formation of a pore, called the translocon, in host membrane (PubMed:10545510, PubMed:17367391, PubMed:34809452). Interacts with IpaC/SctB (PubMed:10545510, PubMed:17367391, PubMed:7954817). Interacts with the needle tip protein IpaD/SctA (PubMed:17367391, PubMed:24236510). Interacts with the molecular chaperone IpgC, which prevents premature association with IpaC/SctB within the cytoplasm of Shigella cells and protects IpaB/SctE from proteolysis (PubMed:11207551, PubMed:19478065, PubMed:20937829, PubMed:7954817). Interacts with the host protein ICE in the cytoplasm of infected macrophages (PubMed:8670890). Interacts with human MAD2L2 in the G2/M phase of the cell cycle (PubMed:17719540, PubMed:31484720)

Gene: sctE  |  Organism: Shigella flexneri  |  Synonyms: 62 kDa antigen; Invasin IpaB
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top