Recombinant Simian immunodeficiency virus Protein Vpx (vpx), Simian immunodeficiency virus (isolate K78) (SIV-mac) (Simian immunodeficiency virus rhesus monkey), E.coli, N-terminal 6xHis-SUMO-tagged, 29.2kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Simian immunodeficiency virus Protein Vpx (vpx)

Recombinant Simian immunodeficiency virus Protein Vpx (vpx) — Plays a role in nuclear translocation of the viral pre-integration complex (PIC), thus is required for the virus to infect non-dividing cells. Purity >90%.

SKU: BCRECP-004743 Categories: ,
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Product Specifications

Product SkuBCRECP-004743
Product DescriptionRecombinant Simian immunodeficiency virus Protein Vpx (vpx) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 1-112aa. [Accession | P11266].
Uniprot No.P11266
Gene Namesvpx
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-112aa
SpeciesSimian immunodeficiency virus (isolate K78) (SIV-mac) (Simian immunodeficiency virus rhesus monkey)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight29.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Plays a role in nuclear translocation of the viral pre-integration complex (PIC), thus is required for the virus to infect non-dividing cells. Targets specific host proteins for degradation by the 26S proteasome. Acts by associating with the cellular CUL4A-DDB1 E3 ligase complex through direct interaction with host DCAF1.

Biological Context

Subcellular Location: Virion; Host nucleus

Product Specifications

Recombinant Simian immunodeficiency virus Protein Vpx (vpx) is a recombinant protein from Simian immunodeficiency virus (isolate K78) (SIV-mac) (Simian immunodeficiency virus rhesus monkey), expressed in E.coli, covering amino acids 1-112aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 29.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Plays a role in nuclear translocation of the viral pre-integration complex (PIC), thus is required for the virus to infect non-dividing cells (By similarity). Targets specific host proteins for degradation by the 26S proteasome (By similarity). Acts by associating with the cellular CUL4A-DDB1 E3 ligase complex through direct interaction with host DCAF1 (PubMed:29891865). This change in the E3 ligase substrate specificity results in the degradation of host SAMHD1 and TASOR (PubMed:29891865). In turn, SAMHD1 depletion allows viral replication in host myeloid cells by preventing SAMHD1-mediated hydrolysis of intracellular dNTPs necessary for reverse transcription (By similarity). TASOR degradation inactivates the HUSH complex, counteracting the transcriptional repression of proviruses (PubMed:29891865)

Subcellular Location

Virion; Host nucleus

Subunit

Interacts with the P6 region of unprocessed GAG (By similarity). Interacts with host VPRBP/DCAF1, leading to change substrate specificity of the CUL4A-DDB1 E3 ligase complex (By similarity)

Gene: vpx  |  Organism: Simian immunodeficiency virus (isolate Mm251)  |  Synonyms: Viral protein X; X ORF protein

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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