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Recombinant Staphylococcus aureus Alpha-hemolysin (hly)

Recombinant Staphylococcus aureus Alpha-hemolysin (hly) — Alpha-toxin binds to the membrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. Purity >95%.

SKU: BCRECP-002994 Category:
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Product Specifications

Product SkuBCRECP-002994
Product DescriptionRecombinant Staphylococcus aureus Alpha-hemolysin (hly) Protein is expressed from Yeast with C-terminal 6xHis-tagged. It contains 27-319aa. [Accession | Q2G1X0].
Uniprot No.Q2G1X0
Gene Nameshly
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region27-319aa
SpeciesStaphylococcus aureus (strain NCTC 8325 / PS 47)
Tag InfoC-terminal 6xHis-tagged
Molecular weight34.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Alpha-toxin binds to the membrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. Inhibits host neutrophil chemotaxis to the lesion region (Probable). Heptamer oligomerization and pore formation is required for lytic activity.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Staphylococcus aureus Alpha-hemolysin (hly) is a recombinant protein from Staphylococcus aureus (strain NCTC 8325 / PS 47), expressed in Yeast, covering amino acids 27-319aa, with C-terminal 6xHis-tagged tag, molecular weight 34.7kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Alpha-toxin binds to the membrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. Inhibits host neutrophil chemotaxis to the lesion region (Probable). Heptamer oligomerization and pore formation is required for lytic activity (By similarity)

Subcellular Location

Secreted

Subunit

Self-assembles to form first a non-lytic oligomeric intermediate and then, a mushroom-shaped homoheptamer structure of 100 Angstroms in length and up to 100 Angstroms in diameter

Gene: hly  |  Organism: Staphylococcus aureus (strain NCTC 8325 / PS 47)  |  Synonyms: Alpha-toxin

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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