Recombinant Staphylococcus aureus Alpha-hemolysin (hly), Staphylococcus aureus, Yeast, Tag-Free, 34.0kDa, purity >90% as determined by SDS-PAGE.

Recombinant Staphylococcus aureus Alpha-hemolysin (hly)

Recombinant Staphylococcus aureus Alpha-hemolysin (hly) — Alpha-toxin binds to the membrane of eukaryotic cells (particularly red blood cells, RBC) forming pores, resulting in hemolysis, with the release of low-molecular weight molecules leading to eventu… Purity >90%.

SKU: BCRECP-002993 Category:
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Product Specifications

Product SkuBCRECP-002993
Product DescriptionRecombinant Staphylococcus aureus Alpha-hemolysin (hly) Protein is expressed from Yeast with Tag-Free. It contains 27-319aa. [Accession | P09616].
Uniprot No.P09616
Gene Nameshly
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region27-319aa
SpeciesStaphylococcus aureus
Tag InfoTag-Free
Molecular weight34.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

Alpha-toxin binds to the membrane of eukaryotic cells (particularly red blood cells, RBC) forming pores, resulting in hemolysis, with the release of low-molecular weight molecules leading to eventual osmotic RBC lysis. Human RBCs bind much less alpha-toxin than do rabbit RBCs. Heptamer oligomerization and pore formation is required for lytic activity.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Staphylococcus aureus Alpha-hemolysin (hly) is a recombinant protein from Staphylococcus aureus, expressed in Yeast, covering amino acids 27-319aa, with Tag-Free tag, molecular weight 34.0kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Alpha-toxin binds to the membrane of eukaryotic cells (particularly red blood cells, RBC) forming pores, resulting in hemolysis, with the release of low-molecular weight molecules leading to eventual osmotic RBC lysis (PubMed:1587866, PubMed:20624979). Human RBCs bind much less alpha-toxin than do rabbit RBCs (PubMed:1587866, PubMed:20624979). Heptamer oligomerization and pore formation is required for lytic activity (PubMed:1587866, PubMed:20624979)

Subcellular Location

Secreted

Subunit

Self-assembles to first form a non-lytic oligomeric intermediate, and then, a mushroom-shaped homoheptamer structure of 100 Angstroms in length and up to 100 Angstroms in diameter (Probable) (PubMed:8943190). Interacts with human ADAM10; this interaction is required for toxin pore formation, disruption of focal adhesions, and hly-mediated cytotoxicity (PubMed:20624979)

Gene: hly  |  Organism: Staphylococcus aureus  |  Synonyms: Alpha-toxin
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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