Recombinant Staphylococcus aureus Alpha-hemolysin (hly), Staphylococcus aureus, Yeast, Tag-Free, 34.0kDa, purity >90% as determined by SDS-PAGE.

Recombinant Staphylococcus aureus Alpha-hemolysin (hly)

Recombinant Staphylococcus aureus Alpha-hemolysin (hly) — Alpha-toxin binds to the membrane of eukaryotic cells (particularly red blood cells, RBC) forming pores, resulting in hemolysis, with the release of low-molecular weight molecules leading to eventu… Purity >90%.

SKU: BCRECP-002993 Category:
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Product Specifications

Product SkuBCRECP-002993
Product DescriptionRecombinant Staphylococcus aureus Alpha-hemolysin (hly) Protein is expressed from Yeast with Tag-Free. It contains 27-319aa. [Accession | P09616].
Uniprot No.P09616
Gene Nameshly
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region27-319aa
SpeciesStaphylococcus aureus
Tag InfoTag-Free
Molecular weight34.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

Alpha-toxin binds to the membrane of eukaryotic cells (particularly red blood cells, RBC) forming pores, resulting in hemolysis, with the release of low-molecular weight molecules leading to eventual osmotic RBC lysis. Human RBCs bind much less alpha-toxin than do rabbit RBCs. Heptamer oligomerization and pore formation is required for lytic activity.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Staphylococcus aureus Alpha-hemolysin (hly) is a recombinant protein from Staphylococcus aureus, expressed in Yeast, covering amino acids 27-319aa, with Tag-Free tag, molecular weight 34.0kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Alpha-toxin binds to the membrane of eukaryotic cells (particularly red blood cells, RBC) forming pores, resulting in hemolysis, with the release of low-molecular weight molecules leading to eventual osmotic RBC lysis (PubMed:1587866, PubMed:20624979). Human RBCs bind much less alpha-toxin than do rabbit RBCs (PubMed:1587866, PubMed:20624979). Heptamer oligomerization and pore formation is required for lytic activity (PubMed:1587866, PubMed:20624979)

Subcellular Location

Secreted

Subunit

Self-assembles to first form a non-lytic oligomeric intermediate, and then, a mushroom-shaped homoheptamer structure of 100 Angstroms in length and up to 100 Angstroms in diameter (Probable) (PubMed:8943190). Interacts with human ADAM10; this interaction is required for toxin pore formation, disruption of focal adhesions, and hly-mediated cytotoxicity (PubMed:20624979)

Gene: hly  |  Organism: Staphylococcus aureus  |  Synonyms: Alpha-toxin
Key Publications

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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