Recombinant Staphylococcus aureus Dihydrofolate reductase (folA), Staphylococcus aureus (strain N315), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 25.6kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Staphylococcus aureus Dihydrofolate reductase (folA)

Recombinant Staphylococcus aureus Dihydrofolate reductase (folA) — Key enzyme in folate metabolism. Purity >90%.

SKU: BCRECP-002773 Categories: ,
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Product Specifications

Product SkuBCRECP-002773
Product DescriptionRecombinant Staphylococcus aureus Dihydrofolate reductase (folA) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 2-159aa. [Accession | P99079].
Uniprot No.P99079
Gene NamesfolA
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-159aa
SpeciesStaphylococcus aureus (strain N315)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight25.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCell Biology

Function

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.

Biological Context

Pathway: Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1

Product Specifications

Recombinant Staphylococcus aureus Dihydrofolate reductase (folA) is a recombinant protein from Staphylococcus aureus (strain N315), expressed in E.coli, covering amino acids 2-159aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 25.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity)

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1

Gene: folA  |  Organism: Staphylococcus aureus (strain N315)

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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