Recombinant Staphylococcus aureus Iron-regulated surface determinant protein A (isdA), Staphylococcus aureus (strain MSSA476), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 35.1kDa, purity >85% as determined by SDS-PAGE.

Recombinant Staphylococcus aureus Iron-regulated surface determinant protein A (isdA)

Recombinant Staphylococcus aureus Iron-regulated surface determinant protein A (isdA) — Cell wall-anchored surface receptor that participates in the extraction of heme from oxidized methemoglobin/metHb to enable growth on hemoglobin as a sole iron source. Purity >85%.

SKU: BCRECP-003190 Category:
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Product Specifications

Product SkuBCRECP-003190
Product DescriptionRecombinant Staphylococcus aureus Iron-regulated surface determinant protein A (isdA) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 47-316aa. [Accession | Q6GA85].
Uniprot No.Q6GA85
Gene NamesisdA
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region47-316aa
SpeciesStaphylococcus aureus (strain MSSA476)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight35.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Cell wall-anchored surface receptor that participates in the extraction of heme from oxidized methemoglobin/metHb to enable growth on hemoglobin as a sole iron source. Receives heme from IsdB and transfers it to IsdC. Also plays a role in the inhibition of host immune response.

Biological Context

Subcellular Location: Secreted, cell wall (Peptidoglycan-anchor)

Product Specifications

Recombinant Staphylococcus aureus Iron-regulated surface determinant protein A (isdA) is a recombinant protein from Staphylococcus aureus (strain MSSA476), expressed in E.coli, covering amino acids 47-316aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 35.1kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Cell wall-anchored surface receptor that participates in the extraction of heme from oxidized methemoglobin/metHb to enable growth on hemoglobin as a sole iron source (By similarity). Receives heme from IsdB and transfers it to IsdC (By similarity). Also plays a role in the inhibition of host immune response. Protects S.aureus against the bactericidal protease activity of apolactoferrin. Decreases bacterial cellular hydrophobicity, which renders S.aureus resistant to bactericidal human skin fatty acids as well as to beta-defensins and cathelicidin. Also binds fibronectin and chains B-beta and gamma of fibrinogen, promoting clumping of S.aureus with fibrinogen. Involved in adherence of S.aureus to human desquamated nasal epithelial cells and is required for nasal colonization (By similarity)

Subcellular Location

Secreted, cell wall (Peptidoglycan-anchor)

Subunit

Monomer. Interacts with IsdC (By similarity). Interacts with IsdB (By similarity)

Gene: isdA  |  Organism: Staphylococcus aureus (strain MSSA476)  |  Synonyms: Fur-regulated protein A; Staphylococcal transferrin-binding protein A

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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