Recombinant Staphylococcus aureus Sensor protein kinase walK (walK), partial, Staphylococcus aureus (strain Newman), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 29.3kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Staphylococcus aureus Sensor protein kinase walK (walK), partial

Recombinant Staphylococcus aureus Sensor protein kinase walK (walK), partial — Member of the two-component regulatory system WalK/WalR that regulates genes involved in cell wall metabolism, virulence regulation, biofilm production, oxidative stress resistance and antibiotic r… Purity >85%.

SKU: BCRECP-004747 Categories: , ,
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Product Specifications

Product SkuBCRECP-004747
Product DescriptionRecombinant Staphylococcus aureus Sensor protein kinase walK (walK) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 382-600aa. [Accession | A6QD58].
Uniprot No.A6QD58
Gene NameswalK
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region382-600aa
SpeciesStaphylococcus aureus (strain Newman)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight29.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Member of the two-component regulatory system WalK/WalR that regulates genes involved in cell wall metabolism, virulence regulation, biofilm production, oxidative stress resistance and antibiotic resistance via direct or indirect regulation of autolysins. Functions as a sensor protein kinase which is autophosphorylated at a histidine residue in the dimerization domain and transfers its phosphate group to the conserved aspartic acid residue in the regulatory domain of WalR. In turn, WalR binds to the upstream promoter regions of the target genes to positively and negatively regulate their expression.

Biological Context

Subcellular Location: Cell membrane (Multi-pass membrane protein)

Product Specifications

Recombinant Staphylococcus aureus Sensor protein kinase walK (walK), partial is a recombinant protein from Staphylococcus aureus (strain Newman), expressed in E.coli, covering amino acids 382-600aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 29.3kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Member of the two-component regulatory system WalK/WalR that regulates genes involved in cell wall metabolism, virulence regulation, biofilm production, oxidative stress resistance and antibiotic resistance via direct or indirect regulation of autolysins. Functions as a sensor protein kinase which is autophosphorylated at a histidine residue in the dimerization domain and transfers its phosphate group to the conserved aspartic acid residue in the regulatory domain of WalR. In turn, WalR binds to the upstream promoter regions of the target genes to positively and negatively regulate their expression

Subcellular Location

Cell membrane (Multi-pass membrane protein)

Subunit

Forms homodimers. Forms homooligomers

Gene: walK  |  Organism: Staphylococcus aureus (strain Newman)

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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