Recombinant Staphylococcus aureus Sensor protein kinase walK (walK), partial, Staphylococcus aureus (strain Newman), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 29.3kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Staphylococcus aureus Sensor protein kinase walK (walK), partial

Recombinant Staphylococcus aureus Sensor protein kinase walK (walK), partial — Member of the two-component regulatory system WalK/WalR that regulates genes involved in cell wall metabolism, virulence regulation, biofilm production, oxidative stress resistance and antibiotic r… Purity >85%.

SKU: BCRECP-004747 Categories: , ,
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Product Specifications

Product SkuBCRECP-004747
Product DescriptionRecombinant Staphylococcus aureus Sensor protein kinase walK (walK) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 382-600aa. [Accession | A6QD58].
Uniprot No.A6QD58
Gene NameswalK
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region382-600aa
SpeciesStaphylococcus aureus (strain Newman)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight29.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Member of the two-component regulatory system WalK/WalR that regulates genes involved in cell wall metabolism, virulence regulation, biofilm production, oxidative stress resistance and antibiotic resistance via direct or indirect regulation of autolysins. Functions as a sensor protein kinase which is autophosphorylated at a histidine residue in the dimerization domain and transfers its phosphate group to the conserved aspartic acid residue in the regulatory domain of WalR. In turn, WalR binds to the upstream promoter regions of the target genes to positively and negatively regulate their expression.

Biological Context

Subcellular Location: Cell membrane (Multi-pass membrane protein)

Product Specifications

Recombinant Staphylococcus aureus Sensor protein kinase walK (walK), partial is a recombinant protein from Staphylococcus aureus (strain Newman), expressed in E.coli, covering amino acids 382-600aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 29.3kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Member of the two-component regulatory system WalK/WalR that regulates genes involved in cell wall metabolism, virulence regulation, biofilm production, oxidative stress resistance and antibiotic resistance via direct or indirect regulation of autolysins. Functions as a sensor protein kinase which is autophosphorylated at a histidine residue in the dimerization domain and transfers its phosphate group to the conserved aspartic acid residue in the regulatory domain of WalR. In turn, WalR binds to the upstream promoter regions of the target genes to positively and negatively regulate their expression

Subcellular Location

Cell membrane (Multi-pass membrane protein)

Subunit

Forms homodimers. Forms homooligomers

Gene: walK  |  Organism: Staphylococcus aureus (strain Newman)

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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