Recombinant Staphylococcus aureus Staphopain A (sspP), Staphylococcus aureus, E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 27.4kDa, purity >90% as determined by SDS-PAGE.

Recombinant Staphylococcus aureus Staphopain A (sspP)

Recombinant Staphylococcus aureus Staphopain A (sspP) — Cysteine protease that plays an important role in the inhibition of host innate immune response. Purity >90%.

SKU: BCRECP-004489 Category:
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Product Specifications

Product SkuBCRECP-004489
Product DescriptionRecombinant Staphylococcus aureus Staphopain A (sspP) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 215-388aa. [Accession | P81297].
Uniprot No.P81297
Gene NamessspP
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region215-388aa
SpeciesStaphylococcus aureus
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight27.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCell Biology

Function

Cysteine protease that plays an important role in the inhibition of host innate immune response. Cleaves host elastins found in connective tissues, pulmonary surfactant protein A in the lungs, and the chemokine receptor CXCR2 on leukocytes. Proteolytic cleavage of surfactant protein A impairs bacterial phagocytosis by neutrophils while CXCR2 degradation blocks neutrophil activation and chemotaxis.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Staphylococcus aureus Staphopain A (sspP) is a recombinant protein from Staphylococcus aureus, expressed in E.coli, covering amino acids 215-388aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 27.4kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Cysteine protease that plays an important role in the inhibition of host innate immune response. Cleaves host elastins found in connective tissues, pulmonary surfactant protein A in the lungs, and the chemokine receptor CXCR2 on leukocytes (PubMed:23235402, PubMed:3422637). Proteolytic cleavage of surfactant protein A impairs bacterial phagocytosis by neutrophils while CXCR2 degradation blocks neutrophil activation and chemotaxis (PubMed:23235402, PubMed:3422637). Additionally, promotes vascular leakage by activating the plasma kallikerin/kinin system, resulting in hypotension (PubMed:15897280)

Subcellular Location

Secreted

Subunit

In the cytoplasm, prematurely activated/folded ScpA forms a stable non-covalent complex with ScpB

Gene: sspP  |  Organism: Staphylococcus aureus  |  Synonyms: Staphylococcal cysteine proteinase A; Staphylopain A
Key Publications

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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