Recombinant Staphylococcus aureus Staphopain A (sspP), Staphylococcus aureus, E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 27.4kDa, purity >90% as determined by SDS-PAGE.

Recombinant Staphylococcus aureus Staphopain A (sspP)

Recombinant Staphylococcus aureus Staphopain A (sspP) — Cysteine protease that plays an important role in the inhibition of host innate immune response. Purity >90%.

SKU: BCRECP-004489 Category:
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Product Specifications

Product SkuBCRECP-004489
Product DescriptionRecombinant Staphylococcus aureus Staphopain A (sspP) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 215-388aa. [Accession | P81297].
Uniprot No.P81297
Gene NamessspP
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region215-388aa
SpeciesStaphylococcus aureus
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight27.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCell Biology

Function

Cysteine protease that plays an important role in the inhibition of host innate immune response. Cleaves host elastins found in connective tissues, pulmonary surfactant protein A in the lungs, and the chemokine receptor CXCR2 on leukocytes. Proteolytic cleavage of surfactant protein A impairs bacterial phagocytosis by neutrophils while CXCR2 degradation blocks neutrophil activation and chemotaxis.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Staphylococcus aureus Staphopain A (sspP) is a recombinant protein from Staphylococcus aureus, expressed in E.coli, covering amino acids 215-388aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 27.4kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Cysteine protease that plays an important role in the inhibition of host innate immune response. Cleaves host elastins found in connective tissues, pulmonary surfactant protein A in the lungs, and the chemokine receptor CXCR2 on leukocytes (PubMed:23235402, PubMed:3422637). Proteolytic cleavage of surfactant protein A impairs bacterial phagocytosis by neutrophils while CXCR2 degradation blocks neutrophil activation and chemotaxis (PubMed:23235402, PubMed:3422637). Additionally, promotes vascular leakage by activating the plasma kallikerin/kinin system, resulting in hypotension (PubMed:15897280)

Subcellular Location

Secreted

Subunit

In the cytoplasm, prematurely activated/folded ScpA forms a stable non-covalent complex with ScpB

Gene: sspP  |  Organism: Staphylococcus aureus  |  Synonyms: Staphylococcal cysteine proteinase A; Staphylopain A
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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