Recombinant Streptococcus downei Cell surface antigen I/II (spaA), partial — recombinant protein from Streptococcus downei (Streptococcus sobrinus), expressed in E.coli. Purity >90%.
| Product Sku | BCRECP-004457 |
| Product Description | Recombinant Streptococcus downei Cell surface antigen I/II (spaA) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 503-832aa. [Accession | P21979]. |
| Uniprot No. | P21979 |
| Gene Names | spaA |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Expression System | E.coli |
| Expression Region | 503-832aa |
| Species | Streptococcus downei (Streptococcus sobrinus) |
| Tag Info | C-terminal 6xHis-tagged |
| Molecular weight | 42.8kDa |
| Activity | Please contact us to obtain bioactivity data. |
| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose. |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Research Areas | Others |
Subcellular Location: Secreted, cell wall (Peptidoglycan-anchor)
Recombinant Streptococcus downei Cell surface antigen I/II (spaA), partial is a recombinant protein from Streptococcus downei (Streptococcus sobrinus), expressed in E.coli, covering amino acids 503-832aa, with C-terminal 6xHis-tagged tag, molecular weight 42.8kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.
SDS-PAGE: Single band at expected molecular weight confirming purity.
ELISA: Suitable as coating antigen or detection standard.
Western Blot: Compatible with standard Western Blot protocols.
Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.
Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.
Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.
Shelf Life: 12 months from date of receipt when stored as recommended.
Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.
Secreted, cell wall (Peptidoglycan-anchor)
Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.
Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.
Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.
Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.
