Recombinant Streptococcus pneumoniae serotype 2 Pneumolysin (ply), Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466), Yeast, C-terminal 6xHis-tagged, 54.9kDa, purity >90% as determined by SDS-PAGE.

Recombinant Streptococcus pneumoniae serotype 2 Pneumolysin (ply)

Recombinant Streptococcus pneumoniae serotype 2 Pneumolysin (ply) — A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol-containing host membranes. Purity >90%.

SKU: BCRECP-004110 Category:
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Product Specifications

Product SkuBCRECP-004110
Product DescriptionRecombinant Streptococcus pneumoniae serotype 2 Pneumolysin (ply) Protein is expressed from Yeast with C-terminal 6xHis-tagged. It contains 2-471aa. [Accession | Q04IN8].
Uniprot No.Q04IN8
Gene Namesply
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region2-471aa
SpeciesStreptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466)
Tag InfoC-terminal 6xHis-tagged
Molecular weight54.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol-containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus.

Biological Context

Subcellular Location: Secreted; Host cell membrane (Multi-pass membrane protein)

Product Specifications

Recombinant Streptococcus pneumoniae serotype 2 Pneumolysin (ply) is a recombinant protein from Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466), expressed in Yeast, covering amino acids 2-471aa, with C-terminal 6xHis-tagged tag, molecular weight 54.9kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol-containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation

Subcellular Location

Secreted; Host cell membrane (Multi-pass membrane protein)

Subunit

Elongated monomers align along their lengths, indicating intersubunit contacts and suggesting the prepore structure (PubMed:15851031, PubMed:26333773, PubMed:26403197, PubMed:27796097, PubMed:28323617). Modeling based on cryo-EM shows a homooligomeric pore complex containing 38-44 subunits; when inserted in the host membrane (Probable). The size of isolated pores is detergent-dependent; in amphipol A8-35 homogeneous rings form with 42 subunits (PubMed:28323617)

Gene: ply  |  Organism: Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466)  |  Synonyms: Thiol-activated cytolysin
Key Publications

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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