Recombinant Thermus aquaticus DNA polymerase I, thermostable (polA) (M747K), partial

Recombinant Thermus aquaticus DNA polymerase I, thermostable (polA) (M747K), partial — In addition to polymerase activity, this DNA polymerase exhibits 5′-3′ exonuclease activity. Purity >85%.

SKU: BCRECP-004135 Category:

Product Specifications

Product SkuBCRECP-004135
Product DescriptionRecombinant Thermus aquaticus DNA polymerase I, thermostable (polA) (M747K) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 293-832aa(M747K). [Accession | P19821].
Uniprot No.P19821
Gene NamespolA
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region293-832aa(M747K)
SpeciesThermus aquaticus
Tag InfoN-terminal 6xHis-tagged
Molecular weight64.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasEpigenetics and Nuclear Signaling

Function

In addition to polymerase activity, this DNA polymerase exhibits 5'-3' exonuclease activity. Unlikely to have 3'-5' exonuclease activity due to absence of a 3'-5' exonuclease domain (Probable).

Product Specifications

Recombinant Thermus aquaticus DNA polymerase I, thermostable (polA) (M747K), partial is a recombinant protein from Thermus aquaticus, expressed in E.coli, covering amino acids 293-832aa(M747K), with N-terminal 6xHis-tagged tag, molecular weight 64.9kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Protein Biology

Function

In addition to polymerase activity, this DNA polymerase exhibits 5'-3' exonuclease activity (By similarity). Unlikely to have 3'-5' exonuclease activity due to absence of a 3'-5' exonuclease domain (Probable)

Gene: polA  |  Organism: Thermus aquaticus  |  Synonyms: Taq polymerase 1
Key Publications

Frequently Asked Questions

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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