Recombinant Tobacco mosaic virus Replicase large subunit, partial, Tobacco mosaic virus (strain OM) (TMV), E.coli, N-terminal 6xHis-Trx-tagged, 46.7kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Tobacco mosaic virus Replicase large subunit, partial

Recombinant Tobacco mosaic virus Replicase large subunit, partial — Is a methyltransferase active in RNA capping and an RNA helicase. Purity >85%.

SKU: BCRECP-003732 Categories: ,
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Product Specifications

Product SkuBCRECP-003732
Product DescriptionRecombinant Tobacco mosaic virus Replicase large subunit, partial Protein is expressed from E.coli with N-terminal 6xHis-Trx-tagged. It contains 829-1085aa. [Accession | O93058].
Uniprot No.O93058
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region829-1085aa
SpeciesTobacco mosaic virus (strain OM) (TMV)
Tag InfoN-terminal 6xHis-Trx-tagged
Molecular weight46.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Is a methyltransferase active in RNA capping and an RNA helicase. Methyltransferase displays a cytoplasmic capping enzyme activity. This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus.

Product Specifications

Recombinant Tobacco mosaic virus Replicase large subunit, partial is a recombinant protein from Tobacco mosaic virus (strain OM) (TMV), expressed in E.coli, covering amino acids 829-1085aa, with N-terminal 6xHis-Trx-tagged tag, molecular weight 46.7kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Is a methyltransferase active in RNA capping and an RNA helicase. Methyltransferase displays a cytoplasmic capping enzyme activity. This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus. Helicase region probably exhibits NTPase and RNA unwinding activities (Potential). It also acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. May mediate silencing suppression through either inhibition of HEN1-mediated siRNA or siRNA demethylation (By similarity)

Subunit

Heterodimer of a large and a small subunit

Organism: Tobacco mosaic virus (strain OM)  |  Synonyms: 183 kDa protein; RNA-directed RNA polymerase

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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