Recombinant Tobacco mosaic virus Replicase large subunit, partial, Tobacco mosaic virus (strain OM) (TMV), E.coli, N-terminal 6xHis-Trx-tagged, 46.7kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Tobacco mosaic virus Replicase large subunit, partial

Recombinant Tobacco mosaic virus Replicase large subunit, partial — Is a methyltransferase active in RNA capping and an RNA helicase. Purity >85%.

SKU: BCRECP-003732 Categories: ,
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Product Specifications

Product SkuBCRECP-003732
Product DescriptionRecombinant Tobacco mosaic virus Replicase large subunit, partial Protein is expressed from E.coli with N-terminal 6xHis-Trx-tagged. It contains 829-1085aa. [Accession | O93058].
Uniprot No.O93058
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region829-1085aa
SpeciesTobacco mosaic virus (strain OM) (TMV)
Tag InfoN-terminal 6xHis-Trx-tagged
Molecular weight46.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Is a methyltransferase active in RNA capping and an RNA helicase. Methyltransferase displays a cytoplasmic capping enzyme activity. This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus.

Product Specifications

Recombinant Tobacco mosaic virus Replicase large subunit, partial is a recombinant protein from Tobacco mosaic virus (strain OM) (TMV), expressed in E.coli, covering amino acids 829-1085aa, with N-terminal 6xHis-Trx-tagged tag, molecular weight 46.7kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Is a methyltransferase active in RNA capping and an RNA helicase. Methyltransferase displays a cytoplasmic capping enzyme activity. This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus. Helicase region probably exhibits NTPase and RNA unwinding activities (Potential). It also acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. May mediate silencing suppression through either inhibition of HEN1-mediated siRNA or siRNA demethylation (By similarity)

Subunit

Heterodimer of a large and a small subunit

Organism: Tobacco mosaic virus (strain OM)  |  Synonyms: 183 kDa protein; RNA-directed RNA polymerase

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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