,

Recombinant Trachypithecus cristatus Cytochrome c oxidase subunit 7A1, mitochondrial (COX7A1), partial

Recombinant Trachypithecus cristatus Cytochrome c oxidase subunit 7A1, mitochondrial (COX7A1), partial — Component of the mitochondrial respiratory complex IV (CIV, also named cytochrome c oxidase complex), the last enzyme in the mitochondrial electron transport chain which drives oxidative phosp…

SKU: BCRECP-002421 Categories: ,

Product Specifications

Product SkuBCRECP-002421
Product DescriptionRecombinant Trachypithecus cristatus Cytochrome c oxidase subunit 7A1, mitochondrial (COX7A1) Protein is expressed from E.coli with N-terminal 6xHis-KSI-tagged. It contains 22-46aa. [Accession | Q9N234].
Uniprot No.Q9N234
Gene NamesCOX7A1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region22-46aa
SpeciesTrachypithecus cristatus (Silvered leaf-monkey) (Presbytis cristata)
Tag InfoN-terminal 6xHis-KSI-tagged
Molecular weight18.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Component of the mitochondrial respiratory complex IV (CIV, also named cytochrome c oxidase complex), the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The CIV complex is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Acts as an assembly factor that specifically drives the homodimerization of CIV complexes, mediating the formation of mitochondrial respiratory supercomplexes (respirasomes) containing two CIV: supercomplxes with two molecules of CIV show improved activity.

Biological Context

Subcellular Location: Mitochondrion inner membrane (Single-pass membrane protein)
Pathway: Energy metabolism; oxidative phosphorylation

Product Specifications

Recombinant Trachypithecus cristatus Cytochrome c oxidase subunit 7A1, mitochondrial (COX7A1), partial is a recombinant protein from Trachypithecus cristatus (Silvered leaf-monkey) (Presbytis cristata), expressed in E.coli, covering amino acids 22-46aa, with N-terminal 6xHis-KSI-tagged tag, molecular weight 18.3kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Protein Biology

Function

Component of the mitochondrial respiratory complex IV (CIV, also named cytochrome c oxidase complex), the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation (By similarity). The CIV complex is the component of the respiratory chain that catalyzes the reduction of oxygen to water (By similarity). Acts as an assembly factor that specifically drives the homodimerization of CIV complexes, mediating the formation of mitochondrial respiratory supercomplexes (respirasomes) containing two CIV: supercomplxes with two molecules of CIV show improved activity (By similarity). Despite being highly expressed in brown adipose tissue, not required for thermogenesis (By similarity)

Subcellular Location

Mitochondrion inner membrane (Single-pass membrane protein)

Subunit

Component of the complex IV (CIV, cytochrome c oxidase), a multisubunit enzyme composed of 14 subunits. The complex is composed of a catalytic core of 3 subunits MT-CO1, MT-CO2 and MT-CO3, encoded in the mitochondrial DNA, and 11 supernumerary subunits COX4I1 (or COX4I2), COX5A, COX5B, COX6A2 (or COX6A1), COX6B1 (or COX6B2), COX6C, COX7A1 (or COX7A2), COX7B, COX7C, COX8B and COXFA4, which are encoded in the nuclear genome. The complex exists as a monomer or a dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with NADH-ubiquinone oxidoreductase (complex I, CI) and ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), resulting in different assemblies (supercomplex SCI(1)III(2)IV(1) and megacomplex MCI(2)III(2)IV(2))

Pathway

Energy metabolism; oxidative phosphorylation

Gene: COX7A1  |  Organism: Trachypithecus cristatus  |  Synonyms: Cytochrome c oxidase subunit VIIa-heart

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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