Recombinant Trichoderma reesei Hydrophobin-2 (hfb2)

Recombinant Trichoderma reesei Hydrophobin-2 (hfb2) — Aerial growth, conidiation, and dispersal of filamentous fungi in the environment rely upon a capability of their secreting small amphipathic proteins called hydrophobins (HPBs) with low sequence i… Purity >90%.

SKU: BCRECP-002985 Category:

Product Specifications

Product SkuBCRECP-002985
Product DescriptionRecombinant Trichoderma reesei Hydrophobin-2 (hfb2) Protein is expressed from Yeast with N-terminal 6xHis-sumostar-tagged. It contains 16-86aa. [Accession | P79073].
Uniprot No.P79073
Gene Nameshfb2
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region16-86aa
SpeciesHypocrea jecorina (Trichoderma reesei)
Tag InfoN-terminal 6xHis-sumostar-tagged
Molecular weight23.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Aerial growth, conidiation, and dispersal of filamentous fungi in the environment rely upon a capability of their secreting small amphipathic proteins called hydrophobins (HPBs) with low sequence identity. Class I can self-assemble into an outermost layer of rodlet bundles on aerial cell surfaces, conferring cellular hydrophobicity that supports fungal growth, development and dispersal; whereas Class II form highly ordered films at water-air interfaces through intermolecular interactions but contribute nothing to the rodlet structure (Probable). Hbf2 is a class II hydrophobin that is involved in sporulation.

Biological Context

Subcellular Location: Secreted; Spore wall; Secreted, cell wall

Product Specifications

Recombinant Trichoderma reesei Hydrophobin-2 (hfb2) is a recombinant protein from Hypocrea jecorina (Trichoderma reesei), expressed in Yeast, covering amino acids 16-86aa, with N-terminal 6xHis-sumostar-tagged tag, molecular weight 23.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Protein Biology

Function

Aerial growth, conidiation, and dispersal of filamentous fungi in the environment rely upon a capability of their secreting small amphipathic proteins called hydrophobins (HPBs) with low sequence identity. Class I can self-assemble into an outermost layer of rodlet bundles on aerial cell surfaces, conferring cellular hydrophobicity that supports fungal growth, development and dispersal; whereas Class II form highly ordered films at water-air interfaces through intermolecular interactions but contribute nothing to the rodlet structure (Probable). Hbf2 is a class II hydrophobin that is involved in sporulation (PubMed:16243453, PubMed:9346297)

Subcellular Location

Secreted; Spore wall; Secreted, cell wall

Subunit

Homodimer (PubMed:17636262). Homodimers further self-assemble to form highly ordered films at water-air interfaces through intermolecular interactions (PubMed:17636262)

Gene: hfb2  |  Organism: Trichoderma reesei  |  Synonyms: Hydrophobin II
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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