Recombinant Vaccinia virus B5R (B5R) Protein, Vaccinia virus, in vitro E.coli expression system, N-terminal 10xHis-tagged, 41.2kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Vaccinia virus B5R (B5R) Protein

Recombinant Vaccinia virus B5R (B5R) Protein — Plays a role in the dissolution of the outermost membrane of extracellular enveloped virions (EV) to allow virion entry into host cells. Purity >85%.

SKU: BCRECP-00361TM Categories: ,
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Product Specifications

Uniprot No.Q80KX4
Gene NamesB5R
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-317aa
SpeciesVaccinia virus
Tag InfoN-terminal 10xHis-tagged
Molecular weight41.2kDa
ActivityNot Test
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Plays a role in the dissolution of the outermost membrane of extracellular enveloped virions (EV) to allow virion entry into host cells. Also participates in wrapping mature virions (MV) to form enveloped virions (EV).

Biological Context

Subcellular Location: Host Golgi apparatus, host trans-Golgi network; Virion membrane (Single-pass type I membrane protein)

Product Specifications

Recombinant Vaccinia virus B5R (B5R) Protein is a recombinant protein from Vaccinia virus, expressed in in vitro E.coli expression system, covering amino acids 1-317aa, with N-terminal 10xHis-tagged tag, molecular weight 41.2kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Plays a role in the dissolution of the outermost membrane of extracellular enveloped virions (EV) to allow virion entry into host cells. Also participates in wrapping mature virions (MV) to form enveloped virions (EV)

Subcellular Location

Host Golgi apparatus, host trans-Golgi network; Virion membrane (Single-pass type I membrane protein)

Subunit

Interacts with OPG161; this interaction is required for efficient targeting of OPG161 and OPG190 into enveloped virions. Interacts with OPG162; this interaction is required for the correct glycosylation, trafficking and stability of OPG162 and OPG190 incorporation into extracellular enveloped virions. Interacts with envelope phospholipase OPG057

Gene: B5R  |  Organism: Vaccinia virus  |  Synonyms: Plaque-size/host range protein

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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