Recombinant Vaccinia virus Protein L1 (L1R), partial, Vaccinia virus (strain Copenhagen) (VACV), Baculovirus, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 23.2kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Vaccinia virus Protein L1 (L1R), partial

Recombinant Vaccinia virus Protein L1 (L1R), partial — Component of the entry fusion complex (EFC), which consists of 11 proteins. Purity >85%.

SKU: BCRECP-003228 Categories: ,
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Product Specifications

Product SkuBCRECP-003228
Product DescriptionRecombinant Vaccinia virus Protein L1 (L1R) Protein is expressed from Baculovirus with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 2-183aa. [Accession | P20540].
Uniprot No.P20540
Gene NamesL1R
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemBaculovirus
Expression Region2-183aa
SpeciesVaccinia virus (strain Copenhagen) (VACV)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight23.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research Areasothers

Function

Component of the entry fusion complex (EFC), which consists of 11 proteins. During cell infection, this complex mediates entry of the virion core into the host cytoplasm by a two-step mechanism consisting of lipid mixing of the viral and cellular membranes and subsequent pore formation.

Biological Context

Subcellular Location: Virion membrane (Single-pass membrane protein)

Product Specifications

Recombinant Vaccinia virus Protein L1 (L1R), partial is a recombinant protein from Vaccinia virus (strain Copenhagen) (VACV), expressed in Baculovirus, covering amino acids 2-183aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 23.2kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Component of the entry fusion complex (EFC), which consists of 11 proteins. During cell infection, this complex mediates entry of the virion core into the host cytoplasm by a two-step mechanism consisting of lipid mixing of the viral and cellular membranes and subsequent pore formation

Subcellular Location

Virion membrane (Single-pass membrane protein)

Subunit

Component of the entry fusion complex (EFC) composed of OPG053, OPG076, OPG086, OPG094, OPG095, OPG099, OPG107, OPG143, OPG104, OPG147 and OPG155. Except for OPG095 and OPG053, each of the EFC proteins is required for assembly or stability of the complex

Gene: OPG099  |  Organism: Vaccinia virus (strain Copenhagen)  |  Synonyms: EFC-associated protein OPG095; Protein L1; Virion membrane protein M25

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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