Recombinant Variola virus 14 kDa fusion protein (A27L), Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus), E.coli, N-terminal 6xHis-tagged, 16.6kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Variola virus 14 kDa fusion protein (A27L)

Recombinant Variola virus 14 kDa fusion protein (A27L) — Structural protein involved in the envelopment of mature virion (MV) to form the wrapped virion (WV). Purity >90%.

SKU: BCRECP-001987 Categories: ,
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Product Specifications

Product SkuBCRECP-001987
Product DescriptionRecombinant Variola virus 14 kDa fusion protein (A27L) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-110aa. [Accession | P33816].
Uniprot No.P33816
Gene NamesA27L
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-110aa
SpeciesVariola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus)
Tag InfoN-terminal 6xHis-tagged
Molecular weight16.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Structural protein involved in the envelopment of mature virion (MV) to form the wrapped virion (WV). The wrapping consists of the addition of Golgi membranes to the mature virion. Participates in mature virion (MV) movement within the infected cell.

Biological Context

Subcellular Location: Virion

Product Specifications

Recombinant Variola virus 14 kDa fusion protein (A27L) is a recombinant protein from Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus), expressed in E.coli, covering amino acids 1-110aa, with N-terminal 6xHis-tagged tag, molecular weight 16.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Structural protein involved in the envelopment of mature virion (MV) to form the wrapped virion (WV). The wrapping consists of the addition of Golgi membranes to the mature virion. Participates in mature virion (MV) movement within the infected cell. May play an indirect role in MV-cell fusion

Subcellular Location

Virion

Subunit

Homohexamers, covalently linked. Interacts with OPG144 and OPG153

Gene: OPG154  |  Organism: Variola virus (isolate Human/India/Ind3/1967)

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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