Recombinant Variola virus Protein OPG161 (OPG161), partial, Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus), Mammalian cell, C-terminal 10xHis-tagged, 15.7kDa, purity >95% as determined by SDS-PAGE.
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Recombinant Variola virus Protein OPG161 (OPG161), partial

Recombinant Variola virus Protein OPG161 (OPG161), partial — Forms a complex with OPG162 and OPG190 to coordinate the incorporation of OPG164 into wrapped enveloped virion (EV) membranes and, subsequently, the production of actin tails. Purity >95%.

SKU: BCRECP-003958 Categories: ,
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Product Specifications

Product SkuBCRECP-003958
Product DescriptionRecombinant Variola virus Protein OPG161 (OPG161) Protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 57-184aa. [Accession | P0DON1].
Uniprot No.P0DON1
Gene NamesOPG161
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region57-184aa
SpeciesVariola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus)
Tag InfoC-terminal 10xHis-tagged
Molecular weight15.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Forms a complex with OPG162 and OPG190 to coordinate the incorporation of OPG164 into wrapped enveloped virion (EV) membranes and, subsequently, the production of actin tails. Therefore plays an essential role in efficient cell-to-cell spread of viral particles.

Biological Context

Subcellular Location: Virion membrane (Single-pass type II membrane protein); Host membrane (Single-pass type II membrane protein)

Product Specifications

Recombinant Variola virus Protein OPG161 (OPG161), partial is a recombinant protein from Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus), expressed in Mammalian cell, covering amino acids 57-184aa, with C-terminal 10xHis-tagged tag, molecular weight 15.7kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Forms a complex with OPG162 and OPG190 to coordinate the incorporation of OPG164 into wrapped enveloped virion (EV) membranes and, subsequently, the production of actin tails. Therefore plays an essential role in efficient cell-to-cell spread of viral particles

Subcellular Location

Virion membrane (Single-pass type II membrane protein); Host membrane (Single-pass type II membrane protein)

Subunit

Homodimer, disulfide-linked. Interacts with protein OPG190. Interacts (via C-terminus) with protein OPG164. Interacts with OPG162

Gene: OPG161  |  Organism: Variola virus (isolate Human/India/Ind3/1967)

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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