Recombinant Vesicular stomatitis Indiana virus Matrix protein (M), Vesicular stomatitis Indiana virus (strain San Juan) (VSIV), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 33.5kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Vesicular stomatitis Indiana virus Matrix protein (M)

Recombinant Vesicular stomatitis Indiana virus Matrix protein (M) — Forms a double layer around the helical nucleocapsid, the inner matrix layer binding to the N helix and the outer matrix layer binding to the envelope glycoprotein. Purity >85%.

SKU: BCRECP-003331 Categories: ,
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Product Specifications

Product SkuBCRECP-003331
Product DescriptionRecombinant Vesicular stomatitis Indiana virus Matrix protein (M) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-229aa. [Accession | P03519].
Uniprot No.P03519
Gene NamesM
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-229aa
SpeciesVesicular stomatitis Indiana virus (strain San Juan) (VSIV)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight33.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasTags & Cell Markers

Function

Forms a double layer around the helical nucleocapsid, the inner matrix layer binding to the N helix and the outer matrix layer binding to the envelope glycoprotein. Plays a major role in assembly and budding of virion, by recruiting cellular partners of the ESCRT complexes that play a key role in releasing the budding particle from the host membrane (Probable). Condensates the ribonucleocapsid core during virus assembly.

Product Specifications

Recombinant Vesicular stomatitis Indiana virus Matrix protein (M) is a recombinant protein, expressed in E.coli, covering amino acids 1-229aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 33.5kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Forms a double layer around the helical nucleocapsid, the inner matrix layer binding to the N helix and the outer matrix layer binding to the envelope glycoprotein (PubMed:35476516, PubMed:35970826). Plays a major role in assembly and budding of virion, by recruiting cellular partners of the ESCRT complexes that play a key role in releasing the budding particle from the host membrane (Probable) (PubMed:15220457, PubMed:16188963, PubMed:22190013). Condensates the ribonucleocapsid core during virus assembly (PubMed:35970826). Inhibits the host mRNA nuclear export thereby inducing the shut off of cellular transcription and preventing the interferon signaling and the establishment of antiviral state in infected cells (PubMed:15629720, PubMed:33849972). This shutoff presumably inhibits interferon signaling and thus establishment of antiviral state in virus infected cells (PubMed:33849972). Induces cell-rounding, cytoskeleton disorganization and apoptosis in infected cell (PubMed:15629720). Inhibits host transcription, possibly through interaction with host DNA repair factor IIH/TFIIH GTF2H5 subunit (PubMed:28888655)

Gene: M  |  Organism: Vesicular stomatitis Indiana virus (strain San Juan)

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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