Recombinant Vibrio cholerae serotype O1 Cholera enterotoxin subunit B (ctxB), Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961), E.coli, N-terminal 6xHis-tagged, 15.6kDa, purity >90% as determined by SDS-PAGE.

Recombinant Vibrio cholerae serotype O1 Cholera enterotoxin subunit B (ctxB)

Recombinant Vibrio cholerae serotype O1 Cholera enterotoxin subunit B (ctxB) — The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. Purity >90%.

SKU: BCRECP-002478 Category:
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Product Specifications

Product SkuBCRECP-002478
Product DescriptionRecombinant Vibrio cholerae serotype O1 Cholera enterotoxin subunit B (ctxB) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 22-124aa. [Accession | P01556].
Uniprot No.P01556
Gene NamesctxB
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region22-124aa
SpeciesVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Tag InfoN-terminal 6xHis-tagged
Molecular weight15.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself.

Biological Context

Subcellular Location: Secreted; Host cell membrane

Product Specifications

Recombinant Vibrio cholerae serotype O1 Cholera enterotoxin subunit B (ctxB) is a recombinant protein from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961), expressed in E.coli, covering amino acids 22-124aa, with N-terminal 6xHis-tagged tag, molecular weight 15.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself

Subcellular Location

Secreted; Host cell membrane

Subunit

The holotoxin (choleragen) consists of a pentameric ring of B subunits whose central pore is occupied by the A subunit. The A subunit contains two chains, A1 and A2, linked by a disulfide bridge

Gene: ctxB  |  Organism: Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)  |  Synonyms: Cholera enterotoxin B chain; Cholera enterotoxin gamma chain; Choleragenoid
Key Publications

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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