Recombinant Vibrio cholerae serotype O1 Cholera enterotoxin subunit B (ctxB), Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961), E.coli, N-terminal 6xHis-tagged, 15.6kDa, purity >90% as determined by SDS-PAGE.

Recombinant Vibrio cholerae serotype O1 Cholera enterotoxin subunit B (ctxB)

Recombinant Vibrio cholerae serotype O1 Cholera enterotoxin subunit B (ctxB) — The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. Purity >90%.

SKU: BCRECP-002478 Category:
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Product Specifications

Product SkuBCRECP-002478
Product DescriptionRecombinant Vibrio cholerae serotype O1 Cholera enterotoxin subunit B (ctxB) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 22-124aa. [Accession | P01556].
Uniprot No.P01556
Gene NamesctxB
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region22-124aa
SpeciesVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Tag InfoN-terminal 6xHis-tagged
Molecular weight15.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself.

Biological Context

Subcellular Location: Secreted; Host cell membrane

Product Specifications

Recombinant Vibrio cholerae serotype O1 Cholera enterotoxin subunit B (ctxB) is a recombinant protein from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961), expressed in E.coli, covering amino acids 22-124aa, with N-terminal 6xHis-tagged tag, molecular weight 15.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself

Subcellular Location

Secreted; Host cell membrane

Subunit

The holotoxin (choleragen) consists of a pentameric ring of B subunits whose central pore is occupied by the A subunit. The A subunit contains two chains, A1 and A2, linked by a disulfide bridge

Gene: ctxB  |  Organism: Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)  |  Synonyms: Cholera enterotoxin B chain; Cholera enterotoxin gamma chain; Choleragenoid
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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