Recombinant Yersinia enterocolitica Heat-stable enterotoxin B (ystB), Yersinia enterocolitica, E.coli, N-terminal 6xHis-SUMO-tagged, 18.1kDa, purity >85% as determined by SDS-PAGE.

Recombinant Yersinia enterocolitica Heat-stable enterotoxin B (ystB)

Recombinant Yersinia enterocolitica Heat-stable enterotoxin B (ystB) — Toxin which activates the particulate form of guanylate cyclase and increases cyclic GMP levels within the host intestinal epithelial cells. Purity >85%.

SKU: BCRECP-004774 Category:
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Product Specifications

Product SkuBCRECP-004774
Product DescriptionRecombinant Yersinia enterocolitica Heat-stable enterotoxin B (ystB) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 53-71aa. [Accession | P74977].
Uniprot No.P74977
Gene NamesystB
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region53-71aa
SpeciesYersinia enterocolitica
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight18.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Toxin which activates the particulate form of guanylate cyclase and increases cyclic GMP levels within the host intestinal epithelial cells. Could play an important role in pathogenesis.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Yersinia enterocolitica Heat-stable enterotoxin B (ystB) is a recombinant protein from Yersinia enterocolitica, expressed in E.coli, covering amino acids 53-71aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 18.1kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Toxin which activates the particulate form of guanylate cyclase and increases cyclic GMP levels within the host intestinal epithelial cells. Could play an important role in pathogenesis

Subcellular Location

Secreted

Gene: ystB  |  Organism: Yersinia enterocolitica  |  Synonyms: Y-STB

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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