Recombinant Yersinia pestis bv. Antiqua Small heat shock protein ibpA (ibpA), Yersinia pestis bv. Antiqua (strain Nepal516), E.coli, N-terminal 6xHis-tagged, 21.6kDa, purity >85% as determined by SDS-PAGE.

Recombinant Yersinia pestis bv. Antiqua Small heat shock protein ibpA (ibpA)

Recombinant Yersinia pestis bv. Antiqua Small heat shock protein ibpA (ibpA) — Associates with aggregated proteins, together with IbpB, to stabilize and protect them from irreversible denaturation and extensive proteolysis during heat shock and oxidative stress. Purity >85%.

SKU: BCRECP-003051 Category:
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Product Specifications

Product SkuBCRECP-003051
Product DescriptionRecombinant Yersinia pestis bv. Antiqua Small heat shock protein ibpA (ibpA) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-137aa. [Accession | Q1CD74].
Uniprot No.Q1CD74
Gene NamesibpA
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-137aa
SpeciesYersinia pestis bv. Antiqua (strain Nepal516)
Tag InfoN-terminal 6xHis-tagged
Molecular weight21.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research Areasothers

Function

Associates with aggregated proteins, together with IbpB, to stabilize and protect them from irreversible denaturation and extensive proteolysis during heat shock and oxidative stress. Aggregated proteins bound to the IbpAB complex are more efficiently refolded and reactivated by the ATP-dependent chaperone systems ClpB and DnaK/DnaJ/GrpE. Its activity is ATP-independent.

Biological Context

Subcellular Location: Cytoplasm

Product Specifications

Recombinant Yersinia pestis bv. Antiqua Small heat shock protein ibpA (ibpA) is a recombinant protein from Yersinia pestis bv. Antiqua (strain Nepal516), expressed in E.coli, covering amino acids 1-137aa, with N-terminal 6xHis-tagged tag, molecular weight 21.6kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Associates with aggregated proteins, together with IbpB, to stabilize and protect them from irreversible denaturation and extensive proteolysis during heat shock and oxidative stress. Aggregated proteins bound to the IbpAB complex are more efficiently refolded and reactivated by the ATP-dependent chaperone systems ClpB and DnaK/DnaJ/GrpE. Its activity is ATP-independent

Subcellular Location

Cytoplasm

Subunit

Monomer. Forms homomultimers of about 100-150 subunits at optimal growth temperatures. Conformation changes to monomers at high temperatures or high ionic concentrations

Gene: ibpA  |  Organism: Yersinia pestis bv. Antiqua (strain Nepal516)  |  Synonyms: 16 kDa heat shock protein A

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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