Recombinant Zaire ebolavirus Matrix protein VP40 (VP40), Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus), Baculovirus, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 39.1kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Zaire ebolavirus Matrix protein VP40 (VP40)

Recombinant Zaire ebolavirus Matrix protein VP40 (VP40) — Plays an essential role virus particle assembly and budding. Purity >85%.

SKU: BCRECP-004737 Categories: , ,
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Product Specifications

Product SkuBCRECP-004737
Product DescriptionRecombinant Zaire ebolavirus Matrix protein VP40 (VP40) Protein is expressed from Baculovirus with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-326aa. [Accession | Q77DJ6].
Uniprot No.Q77DJ6
Gene NamesVP40
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemBaculovirus
Expression Region1-326aa
SpeciesZaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight39.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Plays an essential role virus particle assembly and budding. Acts by interacting with viral ribonucleocapsid and host members of the ESCRT (endosomal sorting complex required for transport) system such as host VPS4, PDCD6IP/ALIX, NEDD4 or TGS101. The interaction with host E3 ubiquitin ligase SMURF2 also facilitates virus budding.

Biological Context

Subcellular Location: Host cytoplasm; Host cell membrane; Virion membrane (Peripheral membrane protein); Host late endosome membrane (Peripheral membrane protein); Host cell membrane (Peripheral membrane protein); Host endomembrane system (Peripheral membrane protein); Secreted, extracellular exosome

Product Specifications

Recombinant Zaire ebolavirus Matrix protein VP40 (VP40) is a recombinant protein from Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus), expressed in Baculovirus, covering amino acids 1-326aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 39.1kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Plays an essential role virus particle assembly and budding. Acts by interacting with viral ribonucleocapsid and host members of the ESCRT (endosomal sorting complex required for transport) system such as host VPS4, PDCD6IP/ALIX, NEDD4 or TGS101. The interaction with host E3 ubiquitin ligase SMURF2 also facilitates virus budding. May play a role in immune cell dysfunction by being packaged into exosomes that can decrease the viability of recipient cells (via RNAi suppression and exosome-bystander apoptosis)

Subcellular Location

Host cytoplasm; Host cell membrane; Virion membrane (Peripheral membrane protein); Host late endosome membrane (Peripheral membrane protein); Host cell membrane (Peripheral membrane protein); Host endomembrane system (Peripheral membrane protein); Secreted, extracellular exosome

Subunit

Homodimer. Homohexamer. Homooctamer. Exists as a dimer until it reorganizes at the plasma membrane into a hexameric form using phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2). Hexamers are critical for budding. Octamers function in genome replication and RNA binding. Interacts with host TSG101. As a homohexamer, interacts with the WW domain 3 of host NEDD4. Interacts with the nucleoprotein/NP. Interacts (via YPx(n)L/I motif) with host PDCD6IP/ALIX; this interaction supports efficient egress of viral particles. Interacts with VP35. Interacts with host ITCH; this interaction is required for efficient egress. Interacts (via PPXY motif) with host SMURF2 (via WW domains); the interaction positively regulates virus budding

Gene: VP40  |  Organism: Zaire ebolavirus (strain Kikwit-95)  |  Synonyms: Ebola VP40; Membrane-associated protein VP40

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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