Recombinant Zaire ebolavirus Matrix protein VP40 (VP40), Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus), Baculovirus, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 39.1kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Zaire ebolavirus Matrix protein VP40 (VP40)

Recombinant Zaire ebolavirus Matrix protein VP40 (VP40) — Plays an essential role virus particle assembly and budding. Purity >85%.

SKU: BCRECP-004737 Categories: , , ,
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Product Specifications

Product SkuBCRECP-004737
Product DescriptionRecombinant Zaire ebolavirus Matrix protein VP40 (VP40) Protein is expressed from Baculovirus with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-326aa. [Accession | Q77DJ6].
Uniprot No.Q77DJ6
Gene NamesVP40
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemBaculovirus
Expression Region1-326aa
SpeciesZaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight39.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Plays an essential role virus particle assembly and budding. Acts by interacting with viral ribonucleocapsid and host members of the ESCRT (endosomal sorting complex required for transport) system such as host VPS4, PDCD6IP/ALIX, NEDD4 or TGS101. The interaction with host E3 ubiquitin ligase SMURF2 also facilitates virus budding.

Biological Context

Subcellular Location: Host cytoplasm; Host cell membrane; Virion membrane (Peripheral membrane protein); Host late endosome membrane (Peripheral membrane protein); Host cell membrane (Peripheral membrane protein); Host endomembrane system (Peripheral membrane protein); Secreted, extracellular exosome

Product Specifications

Recombinant Zaire ebolavirus Matrix protein VP40 (VP40) is a recombinant protein from Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus), expressed in Baculovirus, covering amino acids 1-326aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 39.1kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Plays an essential role virus particle assembly and budding. Acts by interacting with viral ribonucleocapsid and host members of the ESCRT (endosomal sorting complex required for transport) system such as host VPS4, PDCD6IP/ALIX, NEDD4 or TGS101. The interaction with host E3 ubiquitin ligase SMURF2 also facilitates virus budding. May play a role in immune cell dysfunction by being packaged into exosomes that can decrease the viability of recipient cells (via RNAi suppression and exosome-bystander apoptosis)

Subcellular Location

Host cytoplasm; Host cell membrane; Virion membrane (Peripheral membrane protein); Host late endosome membrane (Peripheral membrane protein); Host cell membrane (Peripheral membrane protein); Host endomembrane system (Peripheral membrane protein); Secreted, extracellular exosome

Subunit

Homodimer. Homohexamer. Homooctamer. Exists as a dimer until it reorganizes at the plasma membrane into a hexameric form using phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2). Hexamers are critical for budding. Octamers function in genome replication and RNA binding. Interacts with host TSG101. As a homohexamer, interacts with the WW domain 3 of host NEDD4. Interacts with the nucleoprotein/NP. Interacts (via YPx(n)L/I motif) with host PDCD6IP/ALIX; this interaction supports efficient egress of viral particles. Interacts with VP35. Interacts with host ITCH; this interaction is required for efficient egress. Interacts (via PPXY motif) with host SMURF2 (via WW domains); the interaction positively regulates virus budding

Gene: VP40  |  Organism: Zaire ebolavirus (strain Kikwit-95)  |  Synonyms: Ebola VP40; Membrane-associated protein VP40

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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