Severe acute respiratory syndrome coronavirus 2 Replicase polyprotein 1ab (rep), partial Recombinant Protein, C-His, Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2), Baculovirus, C-terminal 6xHis-tagged, 70.0 kDa, purity >≥ 85% as determined by SDS-PAGE.

Severe acute respiratory syndrome coronavirus 2 Replicase polyprotein 1ab (rep), partial Recombinant Protein, C-His

SKU: BCACP-622305 Category:
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Product SKUBCACP-622305
Product DescriptionSevere acute respiratory syndrome coronavirus 2 Replicase polyprotein 1ab (rep), partial Recombinant Protein is expressed from Baculovirus with C-terminal 6xHis-tagged. It contains 5325-5925aa. [Accession | P0DTD1].
Uniprot No.P0DTD1
Gene Namesrep
Protein LengthPartial
Purity≥ 85% as determined by SDS-PAGE.
Expression SystemBaculovirus
Expression Region5325-5925aa
SpeciesSevere acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
Tag InfoC-terminal 6xHis-tagged
Molecular weight70.0 kDa
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative Names(pp1ab)(ORF1ab polyprotein)(Leader protein)(Non-structural protein 1)(nsp1)(nsp2)(p65 homolog)(Non-structural protein 3)(nsp3)(PL2-PRO)(Papain-like proteinase)(PL-PRO)(nsp4)(3CL-PRO)(3CLp)(Main protease)(Mpro)(Non-structural protein 5)(nsp5)(SARS coronavirus main proteinase)(nsp6)(nsp7)(nsp8)(nsp9)(nsp10)(Growth factor-like peptide)(GFL)(Pol)(RdRp)(Non-structural protein 12)(nsp12)(Hel)(Non-structural protein 13)(nsp13)(ExoN)(Guanine-N7 methyltransferase)(Non-structural protein 14)(nsp14)(NendoU)(Non-structural protein 15)(nsp15)(Non-structural protein 16)(nsp16)

Severe acute respiratory syndrome coronavirus 2 Replicase polyprotein 1ab (rep), partial Recombinant Protein is expressed from Baculovirus with C-terminal 6xHis-tagged. It contains 5325-5925aa. [Accession | P0DTD1].

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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