Recombinant Rat Cytoglobin (Cygb), Rattus norvegicus (Rat), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 28.9kDa, purity >90% as determined by SDS-PAGE.

Recombinant Rat Cytoglobin (Cygb)

Recombinant Rat Cytoglobin (Cygb) — Probable multifunctional globin with a hexacoordinated heme iron required for the catalysis of various reactions depending on redox condition of the cell as well as oxygen availability. Purity >90%.

SKU: BCRECP-002489 Category:
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Product Specifications

Product SkuBCRECP-002489
Product DescriptionRecombinant Rat Cytoglobin (Cygb) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-190aa. [Accession | Q921A4].
Uniprot No.Q921A4
Gene NamesCygb
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-190aa
SpeciesRattus norvegicus (Rat)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight28.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCardiovascular

Function

Probable multifunctional globin with a hexacoordinated heme iron required for the catalysis of various reactions depending on redox condition of the cell as well as oxygen availability. Has a nitric oxide dioxygenase (NOD) activity and is most probably involved in cell-mediated and oxygen-dependent nitric oxide consumption. By scavenging this second messenger may regulate several biological processes including endothelium-mediated vasodilation and vascular tone.

Biological Context

Subcellular Location: Cytoplasm; Nucleus
Tissue Specificity: Widely expressed (at protein level)

Product Specifications

Recombinant Rat Cytoglobin (Cygb) is a recombinant protein from Rattus norvegicus (Rat), expressed in E.coli, covering amino acids 1-190aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 28.9kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Probable multifunctional globin with a hexacoordinated heme iron required for the catalysis of various reactions depending on redox condition of the cell as well as oxygen availability (PubMed:28393874). Has a nitric oxide dioxygenase (NOD) activity and is most probably involved in cell-mediated and oxygen-dependent nitric oxide consumption (PubMed:28393874). By scavenging this second messenger may regulate several biological processes including endothelium-mediated vasodilation and vascular tone (PubMed:28393874). Under normoxic conditions functions as a nitric oxide dioxygenase (NOD) but under hypoxic conditions the globin may switch its function to that of a nitrite (NO2) reductase (NiR), generating nitric oxide. Could also have peroxidase and superoxide dismutase activities, detoxifying reactive oxygen species and protecting cells against oxidative stress. Also binds dioxygen with low affinity and could function as an oxygen sensor but has probably no function as a respiratory oxygen carrier (By similarity)

Subcellular Location

Cytoplasm; Nucleus

Tissue Specificity

Widely expressed (at protein level)

Subunit

Monomeric. Homodimer; disulfide-linked in vitro. Also homooligomeric in vitro

Gene: Cygb  |  Organism: Rattus norvegicus  |  Synonyms: Nitric oxygen dioxygenase CYGB; Nitrite reductase CYGB; Pseudoperoxidase CYGB; Stellate cell activation-associated protein; Superoxide dismutase CYGB

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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